垂体肿瘤转化基因1在雄激素非依赖性前列腺癌发生过程中的表达变化  

Expression of pituitary tumor-transforming gene 1 during the development of androgen-independent prostate cancer

作　　者：曹希亮[1] 宋晓明[1] 于文朝 陈永强[2] 魏洋洋[1] 刘永亮[1] 鲁可权[1] 高江平[3] 

机构地区：[1]蚌埠医学院附属淮海医院/解放军97医院泌尿外科,江苏徐州221004 [2]蚌埠医学院附属淮海医院/解放军97医院肿瘤治疗中心,江苏徐州221004 [3]解放军总医院泌尿外科,北京100086

出　　处：《中华男科学杂志》2016年第8期686-691,共6页National Journal of Andrology

基　　金：南京军区医学科技创新课题(11MB006)~~

摘　　要：目的:探讨垂体肿瘤转化基因1(PTTG1)在前列腺癌由激素依赖性转化为激素非依赖性过程中的表达变化。方法:通过在去雄激素培养条件下长期培养雄激素依赖性前列腺癌细胞株LNCa P,建立并验证雄激素非依赖性亚系LNCa P-AI细胞模型,用Western印迹、RT-PCR方法每2周检测在去雄激素培养过程中PTTG1的表达改变。结果:经过3个月培养,成功建立雄激素非依赖性亚系LNCa P-AI细胞模型;在LNCa P细胞中,随着去雄激素培养时间延长,PTTG1的表达水平逐渐升高,基质金属蛋白酶-2(MMP-2)和MMP-9表达有同步升高趋势。结论:PTTG1表达在雄激素非依赖性前列腺癌发生过程中逐渐增强,可能是晚期前列腺癌基因治疗的靶标之一。Objective： To explore the expression of pituitary tumor transforming gene 1（PTTG1） during the transformation of prostate cancer from androgen-dependent（ ADPC） to androgen-independent（ AIPC）. Methods： We established an AIPC cell model LNCaP-AI by culturing the androgen-dependent LNCaP cell line in the hormone-deprived medium for over 3 months. The cell model was verified and the PTTG1 expression in the LNCaP cells was detected by Western blot and RT-PCR during hormone deprivation.Results： The AIPC cell model LNCaP-AI was successfully established. The PTTG1 expression was gradually increased in the LNCaP cells with the prolonged time of hormone deprivation and the expressions of matrix metalloproteinases MMP-2 and-9 were elevated at the same time. Conclusion： The expression of PTTG1 is increased gradually in AIPC,which may be a target of gene therapy for advanced prostate cancer.

关 键 词：前列腺癌 雄激素非依赖性 垂体肿瘤转化基因1 雄激素剥夺治疗 LNCAP细胞 基质金属蛋白酶-2 基质金属蛋白酶-9 

分 类 号：R737.25[医药卫生—肿瘤]