山药多糖对α-葡萄糖苷酶抑制作用动力学研究  

Study on inhibition kinetics of polysaccharide from yam on α-glucosidase

作　　者：李培[1] 

机构地区：[1]蚌埠学院实验与设备管理中心,安徽蚌埠233030

出　　处：《食品与机械》2016年第7期14-17,共4页Food and Machinery

基　　金：安徽省高等学校省级自然科学研究项目(编号:KJ2013Z199)

摘　　要：以山药为材料,经酶解法提取山药多糖,通过紫外扫描和红外光谱鉴别其组分和结构,以PNPG为底物建立酶—抑制剂体外模型,研究山药多糖对α-葡萄糖苷酶的抑制能力,采用双倒数作图法确定其抑制类型。结果表明:紫外扫描显示提取组分不含核酸和蛋白质,红外光谱显示提取组分具有多糖特征吸收峰,酶—抑制剂体外模型结果表明提取的山药多糖对α-葡萄糖苷酶具有较强的抑制作用,其对α-葡萄糖苷酶的抑制作用为竞争性抑制,抑制常数K_i为65.78mg/m L。The research extracted polysaccharide from the Chinese yam by the methods of enzymolysis. UV scanning and infrared spectroscopy were used to identify the components and structure. Using PNPG as the substrate,the enzyme-inhibitor model was used to study the inhibition ability of polysaccharide from the Chinese yam of the α-glucosidase,and the double reciprocal plot method was used to determine the type of inhibition. The UV scanning results showed that the extract does not contain nucleic acids and proteins,and the infrared spectra showed that the extracts had the characteristic absorption peak of polysaccharide. The enzyme-inhibitor model showed that the polysaccharide from the Chinese yam had stronger inhibition effect on the activity of α-glucosidase. The inhibition type was competitive inhibition, with inhibition constant factor K_i of65. 78 mg / m L.

关 键 词：山药 多糖 红外光谱 双倒数作图 竞争性抑制 抑制常数 

分 类 号：TS201.4[轻工技术与工程—食品科学]