鹅去氧胆酸诱导HCT-116细胞凋亡及其作用机制研究  

The effect and mechanism of apoptosis induced by chenodeoxycholic acid in human colon cancer HCT-116 cells

作　　者：郭帅 高艳平 赵亚影 周恩旭[3] 付丽[3] 高慧茹[3] 任元静 赵安芳[3] 

机构地区：[1]河南省平顶山市第二人民医院普外科,467000 [2]河南省驻马店市环境监测站,463000 [3]河南城建学院生命科学与工程学院,河南平顶山467036

出　　处：《重庆医学》2016年第23期3169-3172,共4页Chongqing medicine

基　　金：国家自然科学基金项目(81402309);河南省科技攻关项目(132102310118;142102310120);河南省教育厅科学技术研究重点项目(13A180044)

摘　　要：目的研究鹅去氧胆酸(CDCA)对结肠癌细胞HCT-116的影响,并探讨其作用机制。方法采用体外细胞培养、四甲基偶氮唑蓝(MTT法)、苏木素-伊红染色(HE染色)、吉姆萨染色(Giemsa染色)、吖啶橙/溴化乙锭染色(AO/EB染色)和流式细胞术检测经CDCA诱导处理对结肠癌HCT-116细胞的存活率、形态学变化、细胞周期、线粒体膜电位和细胞凋亡率的影响。结果试验结果表明,CDCA作用于结肠癌细胞HCT-116细胞株48h的半抑制浓度为0.304mmol/L。0.800mmol/L CDCA处理48h后细胞抑制率达88.4%,且表现出细胞体积缩小、细胞核固缩和染色质凝聚等凋亡特征性的形态学改变。流式细胞仪检测结果显示,经CDCA诱导处理后,细胞株细胞周期被阻滞于G2/M期,线粒体膜电位降低,0.400mmol/L CDCA处理48h后HCT-116细胞凋亡率达54.5%。结论 CDCA对人结肠癌HCT116细胞株的抑制作用与其剂量有依赖性,并可能通过线粒体途径诱导人结肠癌细胞HCT-116细胞株凋亡。Objective To evaluate the effects of chenodeoxycholic acid （CDCA） on human colon cancer ceilline and to ex- plore its mechanisms. Methods We used cell culture,MTT assay, HE staining,Giemsa staining,AO/EB staining and flow cytome- try to detect cell survival rate, morphological changes, cell cycle, mitochondrial membrane potential and cell apoptosis rate of HCT116 cells after CDCA treated. Results HCT-116 cells were treated with CDCA for 48 h and its IC50 was 0. 304 mmol/L;the inhibition rate of 88.40/6 was achieved when the concentration was 0. 800 retool/L, showing nuclear chromatin concentration and fragmentation as well as cell shrinkage and other morphological changes characteristic of apoptosis;CDCA arrested cell cycle at G2/M phase and induced mitochondrial membrane potential low. After treated with CDCA（0. 400 mmol/L） for 48 h,cell apoptosis rate was 54.5%. Conclusion CDCA significantly inhibits the growth of HCT-116 cells in a dose-dependent manner and arrested cell cycle at G2/M phase,which indicates apoptosis of HCT-116 ceils could be induced by CDCA via the mitochondrial-dependent pathway.

关 键 词：鹅脱氧胆酸 结肠肿瘤 细胞凋亡 细胞 

分 类 号：R735.34[医药卫生—肿瘤]