沉淀聚合法制备3-吲哚乙酸印迹聚合物微粒及其固相萃取应用  

Preparation of Indole-3-acetic Acid-imprinted Polymer Particulates by Precipitation Polymerization and Their Application in Solid Phase Extraction

作　　者：张铁莉[1] 赫彩霞[1,2] 王磊[1] 

机构地区：[1]唐山师范学院化学系,河北唐山063000 [2]河北科技大学生物科学与工程学院,河北石家庄050018

出　　处：《分析测试学报》2016年第8期962-967,共6页Journal of Instrumental Analysis

基　　金：唐山师范学院团队支撑重点项目(2014D07)

摘　　要：以3-吲哚乙酸（IAA）为模板分子、丙烯酰胺（AA）为功能单体,采用沉淀聚合法制备了IAA印迹聚合物微粒,研究了致孔剂乙腈的用量及IAA的加入量对印迹聚合物结合性能的影响,采用色谱法对其进行评价。结果表明,当乙腈为37.5 m L,IAA为2 mmol（与AA物质的量比为1∶1）时,制得印迹聚合物P7对IAA的亲和力最大（k=4.24）,印迹因子（IF）为14.1,其对IAA展示了最显著的特异识别能力。将P7作为固相萃取柱的填料,研究了分子印迹固相萃取IAA的方法,测得绿豆芽样品的加标回收率为90.6%～92.6%。结果表明,以AA为功能单体沉淀聚合法制备的IAA印迹聚合物颗粒适合作为固相萃取柱填料,可实现实际样品中IAA的选择性分离净化。该文提出的采用适当增大模板分子加入量的方法是制备高亲和力与高选择性印迹聚合物的一条新途径。Indole-3-acetic acid （IAA）-imprinted polymer mieroparticles were synthesized using IAA as template, acrylamide （AA） as functional monomer and ethylene glycol dimethacrylate as erosslink- er in aeetonitrile porogen via precipitation polymerization. Effects of amounts of IAA and acetonitrile utilized in the polymerization reaction on the binding performances of IAA-imprinted polymers were e- valuated by high-performance liquid chromatography （HPLC）. The results showed that the IAA-im- printed polymer P7 with the highest affinity （k = 4. 24） and imprinting factor（ IF = 14. 1 ） for IAA was obtained when the molar ratio of IAA to AA 1 ： 1 was adopted with 2 mmol of IAA in 37.5 mL of ace- tonitrile porogen. Furthermore, P7 was chosen as the packing material to make the molecularly im- printed solid phase extraction （MISPE） column, and the MISPE method was developed for the purifi- cation of IAA . Under the optimized extraction conditions, the mungbean sprout samples spiked with IAA were extracted, and the recoveries of IAA ranged from 90.6% to 92.6% . The results demon- strated that the IAA-imprinted polymer particulates prepared using AA as the functional monomer by precipitation polymerization could be used as the adsorbent of solid phase extraction column for the se- lective separation and purification of IAA in real samples. A new route was proposed in this paper to synthesize an imprinted polymer with high affinity and high selectivity by means of increasing amount of template appropriately in the pre-polymerization mixture.

关 键 词：3-吲哚乙酸 沉淀聚合 分子印迹聚合物 固相萃取 高效液相色谱(HPLC) 绿豆芽 

分 类 号：O657.72[理学—分析化学] O629.8[理学—化学]