四通道色谱分离仪净化/气相色谱-质谱法测定烟叶中的多环芳烃  

Determination of 16 Polycyclic Aromatic Hydrocarbons in Tobacco( Nicotiana Tabacum L.) by Four-channel Chromatograph Purification Coupled with Gas Chromatography-Mass Spectrometry

作　　者：马军[1,2,3] 滕应[2] 陆引罡[1] 甘信宏 任文杰[2] 马文亭[2] 

机构地区：[1]贵州大学农学院,贵州贵阳550025 [2]中国科学院南京土壤研究所土壤环境与污染修复重点实验室,江苏南京210008 [3]铜仁学院材料与化学工程学院,贵州铜仁554300

出　　处：《分析测试学报》2016年第8期968-973,共6页Journal of Instrumental Analysis

基　　金：环保公益性行业科研专项项目(201109018)

摘　　要：建立了同时检测新鲜烟叶中16种多环芳烃（PAHs）的四通道色谱分离仪净化/气相色谱-质谱（GC-MS）分析方法。样品经二氯甲烷索氏提取,四通道色谱分离仪净化,GC-MS选择离子监测模式（SIM）测定和外标法定量,重点考察了新鲜烟叶提取液的分离净化条件。结果表明：16种PAHs类化合物在10～1 000μg/kg范围内定量离子的峰面积与质量浓度呈较好的线性关系（r〉0.99）。仪器检出限（LOD）为0.35～1.75μg/kg,方法检出限（MDLs）为1.01～2.87μg/kg。在10,50,200μg/kg 3个浓度水平下的加标回收率为81.0%～107.8%,相对标准偏差（RSD,n=6）为2.7%～8.0%。该方法简便、快捷、可操作性强、应用范围广,适合于烟草等作物中PAHs类化合物的分析检测。A method for the simultaneous determination of 16 polycyclic aromatic hydrocarbons （PAHs） in tobacco by four-channel chromatograph purification coupled with gas chromatography - mass spectrometry（ GC -MS） was developed. The tobacco samples were extracted by the method of soxhlet extraction with dichloromethane, and the extract was cleaned with four-channel purification chromatograph. The eluent were determined by GC -MS in SIM mode, and quantitated by a method of external standard. The procedure of separation and purification for fresh tobacco extraction was op- timized. The results indicated the calibration curves of 16 PAHs had good linear relationships in the range of 10- 1 000 μg/kg（r 〉 0.99）. The detection of limits（LODs） and method detection limits （MDLs） were 0.35 - 1.75 μg/kg and 1.01 - 2. 87 μg/kg, respectively. The recoveries at three spiked concentration levels were in the range of 81.0% - 107.8% with relative standard deviations （ RSD, n = 6） of 2.7% - 8.0%. The method is suitable for the determination of PAHs in tobacco or other crops with easy operation, rapidness, high repeatability and strong practicability.

关 键 词：四通道色谱分离仪 气相色谱-质谱 多环芳烃 净化 烟叶 

分 类 号：O657.63[理学—分析化学] O625.15[理学—化学]