表没食子儿茶素没食子酸酯(EGCG)体外抗日本血吸虫尾蚴作用的研究  

In vitro effects of EGCG on Schistosoma japonicum cercariae

作　　者：冯金梅[1] 袁发浒[1] 刘锴[1] 张泽华[1] 胡松[1] 邱文洪[1] Stephen Hsu 宋文剑[1] 

机构地区：[1]江汉大学医学院病原生物学教研室,湖北武汉430056

出　　处：《中国病原生物学杂志》2016年第7期629-633,共5页Journal of Pathogen Biology

基　　金：国家自然科学基金项目(No.31501854);湖北省卫生计生委血防专项(No.WJ2015XB012);江汉大学高层次人才科研启动项目(No.06000025);湖北省自然科学基金面上项目(No.2014CFB460)

摘　　要：目的观察表没食子儿茶素-3-没食子酸酯(Epigallocatechin-3-gallate,EGCG)对日本血吸虫尾蚴的体外杀伤作用及其超微组织结构的变化,探讨EGCG预防日本血吸虫尾蚴感染的效果,为进一步开展血吸虫尾蚴感染预防措施的研究提供实验数据。方法将日本血吸虫尾蚴分别置于空白对照组蒸馏水、药物处理组0.2%EGCG和1%EGCG溶液中,各处理0、30和60min,解剖镜下观察并摄像;分别选取以上3组处理30min的尾蚴进行扫描电镜观察。设置蒸馏水对照组、0.1%和1%EGCG溶液处理30min组尾蚴感染小白鼠,饲养43d后解剖小鼠,冲虫,观察感染情况。结果解剖镜下观察,蒸馏水对照组在0min和30min时尾蚴体态柔和,自由运动;60min时尾蚴虫体完整,蜷缩成团,活动停止。0.2%和1%EGCG处理组在30min时尾蚴剧烈运动,尾部急剧摆动、挛缩,体部发生变形,且1%EGCG处理组比0.2%EGCG处理组尾蚴运动更为剧烈,形态变形更明显;60min时,两组尾蚴尾部都已脱落且死亡。扫描电镜观察对照组尾蚴形态修长且虫体完整,口吸盘、头器和腹吸盘的结构正常,体部与尾部连接紧密,尾蚴体被表面光滑,体棘遍布全身,且头部和躯干部体棘的排列和棘的尖端都朝向身体后方。0.2%和1%EGCG处理30min组尾蚴体部高度收缩且皱褶不平,尾蚴口吸盘变形、肿大,腹吸盘肿胀、变窄,其中1%EGCG处理组比0.2%EGCG处理组尾蚴体部的收缩皱褶和腹吸盘变形更明显;两组EGCG处理组尾蚴体部与尾部连接松弛,尾端呈皱褶状变化,其中1%EGCG处理30min组尾蚴的尾部都已脱落,体表体棘散在分布,体棘紊乱、朝向不一,部分体棘脱落。动物感染试验显示对照组和0.1%EGCG处理组尾蚴感染小鼠的感染率均为100%,但0.1%EGCG处理组的减虫率为33.52%;1%EGCG处理组尾蚴感染小鼠的感染率为16.7%,减虫率81.87%。结论 EGCG对日本血吸虫尾蚴具有体外杀伤作用,发生的形态结构可能影响其侵袭宿主皮肤Objectives To investigate whether epigallocatechin-3-gallate（EGCG）kills Schistosoma japonicum cercariae in vitro and its effects on the ultrastructure of those cercariae in order to evaluate whether EGCG protects against infection with S.japonicumcercariae. Methods Cercariae of S.japonicum were placed in distilled water（control group）or different concentrations of EGCG（0.2% and 1%）.Cercariae were treated for 30 and 60min and then observed with a dissecting microscope.Cercariae that were treated for 30 min were also observed using scanning electron microscopy.Male BALB/c mice,6-8 weeks of age,were randomly divided into three groups with six animals each：group I（control group,infected with S.japonicumcercariae treated with 0% EGCG for 30min）,group Ⅱ（infected with S.japonicum cercariae treated with 0.1% EGCG for 30 min）,and group Ⅲ（infected with S.japonicumcercariae treated with 1%EGCG for 30min）.All experimental mice were anesthetized and percutaneously infected with 30 cercariae of S.japonicumthat were placed on shaved abdominal skin.The animals were maintained in conventional animal housing at the Laboratory Animal Center of Jianghan University,China.All mice were given standard rodent food pellets and water ad libitum.At six weeks post-infection,the mice were sacrificed and adult worms of S.japonicum were collected,and the rate of infection,the average worm burden,and the rate of worm clearance were calculated. Results Under a dissecting mi-croscope,S.japonicumcercariae treated with 0% EGCG for 30min（the controls）had a normal morphology and they moved freely,while cercariae treated with 0% EGCG for 60 min were rigid,curled up into a ball,and not moving.Cercariae that were treated with 0.2% or 1% EGCG for 30 min were highly active,their tails lashed rapidly,they twitched,and their bodies were deformed.Compared to cercariae that were treated with 0.2% EGCG for 30 min,cercariae that infected group Ⅲ were more active and morphological changes were more apparent.When cercariae were

关 键 词：血吸虫 日本 尾蚴 EGCG 扫描电镜 

分 类 号：R383.24[医药卫生—医学寄生虫学]