噬菌体展示技术在肿瘤防治过程中的应用  

Advances in the Applications of Phage Display Technology for Cancer Prevention

作　　者：王瀚[1] 赵淑玲[1] 卓平清 

机构地区：[1]陇南师范高等专科学校生命科学与技术系,甘肃陇南742500

出　　处：《德州学院学报》2016年第4期46-50,共5页Journal of Dezhou University

基　　金：陇南师专2014年校级重点科研项目(No.2014LSZK01003)

摘　　要：噬菌体展示肽技术是一种特异性多肽筛选技术,它通过将随机序列的外源DNA片段定向插入至丝状噬菌体的相关基因后,相应的肽序会以融合蛋白的形式展示于噬菌体表面.噬菌体和特异性抗原特异性结合后将其富集后便可准确认识与相应靶位相结合的多肽序列.近年来这种技术以应用到生命科学的多个领域,尤其在抗肿瘤研究过程中,这种技术可用于肿瘤标志物的筛选、肿瘤抗原抗体库的建立、多肽分子探针的筛选等领域.Phage Display Technology has been developed as a powerful tool for selecting peptides from huge random peptide libraries. In this library, each phage clone carries a different foreign DNA insert and therefore displaying a different peptide on its surface. Phage display has been used to allow rapid identification of peptide ligands for a variety of target molecules. Screening libraries of synthetic compounds for substances that bind the target receptor and that might therefore be leads to new biomarkers, antigen epitopes and peptide probes. In recent years, such technology has been widely applied in many fields, es pecially in the tumor research.

关 键 词：噬菌体展示肽库 肿瘤 肿瘤标志物 多肽分子探针 

分 类 号：R730.3[医药卫生—肿瘤]