固体脂质纳米姜黄素通过PI3K/AKT通路对宫颈癌Caski细胞增殖的影响  

Curcumin-loaded solid lipid nanoparticles induces human cervical carcinoma cell line Caski cells growth inhibition by suppressing phosphatidylinositol 3 kinase/protein kinase B signaling

作　　者：丁书军[1] 黄宁[2] 黄丽霞[1] 

机构地区：[1]江西省妇幼保健院检验科,南昌330006 [2]江西省妇幼保健院产前诊断中心,南昌330006

出　　处：《江西医药》2016年第8期753-755,共3页Jiangxi Medical Journal

基　　金：江西省卫生计生委中医药科研课题;编号:2014A031

摘　　要：目的探讨固体脂质纳米姜黄素对宫颈癌Caski细胞增殖的影响。方法采用溶液扩散法制备Cur-SLN,设置CurSLN药物组(药物浓度分别为10、20、40、60μmol/L)、阴性对照组(不加Cur-SLN,仅含正常的Caski细胞)及空白对照组(不含细胞仅加培养液)。分别用MTT法测定细胞生长抑制率,流式细胞术检测细胞凋亡,Western-blot检测各组细胞PI3K、AKT和p-AKT蛋白表达。结果随着Cur-SLN浓度的增加,Caski细胞的抑制率与细胞凋亡明显上升,Caski细胞PI3K、p-AKT蛋白则呈现Cur-SLN浓度依赖性下调表达,AKT蛋白表达无明显变化。10、20、40、60umol/L Cur-SLN处理组、阴性对照组,组间差异均有统计学意义,P<0.05。结论 Cur-SLN抑制PI3K/AKT信号通路激活,诱发细胞凋亡,进而导致Caski细胞增殖抑制,为宫颈癌的临床治疗提供了新的理论依据。Objective To investigate the effect of Curcumin-loaded solid lipid nanoparticles （Cur-SLN） on apoptosis and growth of human cervical carcinoma cell line Caski cells. Methods Preparing Cur-SLN by Solution diffusion method. Caski cells in logarithmic growth phase were treated with different concentrations of Cur-SLN （0、10、20、40、60umol/L） for 24h. MTT and flow cytometric analysis were used to evaluate the proliferation and apoptosis of Caski cells. The expression of phosphatidylinositol 3 ki-nase （PI3K）,p-protein kinase B（p-AKT） and AKT in Caski cells was detected by Western blot. Results Cur-SLN treatment sig-nificantly increased cell apoptosis and decreased the cell proliferation and the expression of PI3K and p-AKT in Caski cells. However,Cur-SLN treatment had no significant influence on the expression of AKT. Conclusion Cur-SLN induces cell apoptosis and growth inhibition in the human cervical carcinoma cell line Caski cells possibly by suppressing PI3K/AKT signaling pathway.

关 键 词：固体脂质纳米粒 姜黄素 细胞凋亡 PI3K/AKT信号通路 

分 类 号：R737.33[医药卫生—肿瘤]