NF-κB在初治急性髓系白血病中的表达与意义  

The expression and significance of NF-kappa B in new diagnosed acute myeloid leukemia

作　　者：魏宇靖[1,2] 刘婷婷[2] 柯波[2] 符环[2] 程洪波[2] 

机构地区：[1]武汉大学基础医学院,武汉430071 [2]江西省人民医院血液内科,南昌330006

出　　处：《江西医药》2016年第8期759-761,共3页Jiangxi Medical Journal

摘　　要：目的应用RT-PCR技术检测NF-κB在初治急性髓系白血病诱导治疗前后表达的情况,研究NF-κB与急性髓性白血病之间的关系,并探讨NF-κB抑制剂用于急性髓系白血病的理论依据。方法应用逆转录聚合酶链式扩增反应(RT-PCR)技术检测初治急性髓系白血病患者诱导治疗前后与对照组骨髓单个核细胞中NF-κB的表达,分析NF-κB与急性髓系白血病之间的关系。结果⑴初治急性髓性白血病患者治疗前的NF-κB表达高于对照组,差异具有统计学意义(P<0.05)。⑵经诱导治疗后达完全缓解的急性髓系白血病患者,其NF-κB的表达明显下降,差异具有统计学意义(P<0.05)。⑶经诱导治疗后未达完全缓解的急性髓系白血病患者,其NF-κB的表达高于对照组与治疗后完全缓解者,差异具有统计学意义(P<0.05)。结论初治急性髓性白血病NF-κB基因明显升高,提示NF-κB的高表达可能是急性髓系白血病重要的致病因素,而经诱导治疗后未达完全缓解的患者与完全缓解患者之间NF-κB基因表达差异显著,提示NF-κB可能是影响急性髓系白血病诱导治疗效果的重要因素之一。Objective To detect NF-κB in new diagnosed acute myeloid leukemia before and after treatment induced expres-sion of NF-κB Relations with acute myeloid leukemia between,and to explore the NF kappa B inhibitor for the theoretical basis of acute myeloid leukemia. Methods By RT-PCR detected NF-κB of bone marrow mononuclear cells in before and after treatment new diagnosed acute myeloid leukemia patients with control group,Analysis of the relationship between NF-nuclear factor kappa B and acute myeloid leukemia. Results ⑴In patients with acute myeloid leukemia before treatment,the expression of NF-kappa B was higher than that of the control group,the difference was statistically significant （P〈0.05）. ⑵After induction therapy of acute myeloid leukemia patients reached complete remission,the expression of NF-kappa B decreased significantly,the difference was statistically significant （P〈0.05）.⑶In patients with acute myeloid leukemia who did not achieve complete remission after induction therapy,the expression of NF-kappa B was higher than that in control group and complete remission after treatment,the difference was statistically significant （P〈0.05）. Conclusion New diagnosed acute myeloid leukemia NF kappa B gene significantly in-creased,suggesting that the NF kappa B expression may be an important etiologic factor for acute myeloid leukemia,After induc-tion therapy,the NF-nuclear factor B gene expression was significantly different between patients with complete remission and complete remission,suggesting that NF-kappa B may be one of the important factors influencing the treatment effect of acute myeloid leukemia.

关 键 词：NF-ΚB 急性髓系白血病 RT-PCR 预后 

分 类 号：R733.7[医药卫生—肿瘤]