老年住院患者肺炎克雷伯菌临床分离株多位点可变数目串联重复序列基因分型特征分析  

Genotyping of Klebsiella Pneumonia Strains Isolated from Eldly Inpatients by Multiple-locus Variable-number Tandem-repeat Analysis

作　　者：张媛媛[1,2] 徐雅萍[3] 杜鹏程[1,2] 强裕俊 张雯[1] 陈晨[2] 于季红[3] 郭军[4,5] 

机构地区：[1]中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室,北京102206 [2]首都医科大学附属北京地坛医院传染病研究所,北京100015 [3]解放军总医院南楼临床部检验科,北京100853 [4]清华大学医学中心北京清华长庚医院呼吸内科,北京102218 [5]解放军总医院南楼临床部呼吸内科,北京100853

出　　处：《中国医学科学院学报》2016年第4期434-437,共4页Acta Academiae Medicinae Sinicae

基　　金：中国博士后科学基金面上资助(2014M562610)~~

摘　　要：目的探讨老年住院患者肺炎克雷伯菌分离株多位点可变数目串联重复序列分析的基因分型特点。方法收集解放军总医院南楼临床部老年住院患者肺炎克雷伯菌临床分离菌株184株,提取基因组DNA,采用多重PCR和毛细管电泳对7个位点进行分析,利用Bio Numerics 5.1软件进行聚类分析。结果 184株肺炎克雷伯菌临床分离株共产生139种基因型,呈现明显的基因多态性;聚类分析结果显示,全部菌株可分为3个基因群,其中Ⅰ群包含93.06%的菌株。结论本医疗中心老年住院患者的肺炎克雷伯菌临床分离株具有明显的优势菌群,其感染来源仍以院内感染为主。Objective To investigate the genotype of klebsiella pneumonia strains isolated from eldly inpatients by multiple-locus variable-number tandem-repeat analysis. Methods Totally 184 klebsiella pneumonia strains,isolated from eldly inpatients,were collected,and their genome DNA were extracted. The polymorphism of 7 variable-number tandem-repeat locus in the DNA samples was analyzed by multiple primers polymerase chain reaction and capillary electrophoresis. The clustering analysis of genotyping was carried out with the Bio Numerics 5. 1 software. Results A total of 139 genotypes were identified in 184 klebsiella pneumonia clinical strains,showing obvious genetic polymorphisms. With clustering analysis of genotypes,all the strains were categorized into three gene clusters（ genogroups Ⅰ,Ⅱ,and Ⅲ）. The genogroup Ⅰ was the biggest cluster,containing 93. 06% of the isolated strains. Conclusion There was a predominant cluster in the klebsiella pneumonia strains isolated from eldly inpatients in our center,and the major source of klebsiella pneumonia infection remained the nosocomial infection.

关 键 词：老年住院患者 感染 肺炎克雷伯菌 多位点可变数目串联重复序列分析 

分 类 号：R183.3[医药卫生—流行病学]