IL-32γ对LX-2细胞表达Ⅰ型胶原的影响  

The effect of IL-32γ on Collagen Ⅰ expression by LX-2 cells

作　　者：潘兴飞[1] 张绍全[2] 杨小安[2] 邹小芳[1] 魏立平[1] 

机构地区：[1]广州医科大学附属第三医院感染科,广州510150 [2]中山大学附属第三医院感染科,广州510630

出　　处：《广东医学》2016年第15期2221-2223,共3页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:81401306);广州医科大学附属第三医院博士启动基金(编号:2014Y02);广东省医学科研基金资助项目(编号:A2014318)

摘　　要：目的研究IL-32γ可否诱导肝星状细胞LX-2表达Ⅰ型胶原(CollagenⅠ)。方法不同浓度的IL-32γ与LX-2共培养,分别收集培养上清液、提取细胞总RNA,real-time PCR检测CollagenⅠmRNA表达水平,ELISA法检测CollagenⅠ蛋白质表达水平。用不同浓度的人重组磷酸化p38α蛋白(recombinant human active p38α蛋白)与LX-2细胞共培养,48 h后收集细胞培养上清液,ELISA法检测CollagenⅠ蛋白质表达水平。再用不同浓度的p38MAPK抑制剂SB203580加入LX-2细胞与IL-32γ共培养的培养液中,分别收集培养上清液,采用ELISA检测CollagenⅠ蛋白质表达水平,同时提取细胞总RNA采用real-time PCR检测CollagenⅠmRNA表达水平。结果 IL-32γ诱导人LX-2细胞表达CollagenⅠ,且其表达水平随IL-32γ浓度的增加而增强;人重组磷酸化p38α蛋白可诱导LX-2细胞表达CollagenⅠ增高,阻断p38MAPK可降低IL-32γ诱导的CollagenⅠ表达。结论 IL-32γ通过活化p38MAPK通路诱导LX-2细胞表达CollagenⅠ,IL-32γ可能通过诱导肝星状细胞表达CollagenⅠ而参与肝纤维化的形成。Objective To investigate whether could IL- 32γ induce Collagen Ⅰ expression in LX- 2 cells.Methods LX- 2 cells were co- cultured with IL- 32γ of different concentrations. Then total RNA was extracted,and the cell supernatant was collected. Collagen Ⅰ RNA expression level was assayed by using real- time PCR. Collagen Ⅰprotein expression level was detected by using ELISA. Subsequently,LX- 2 cells were co- cultured with recombinant Human Active p38α protein of different concentrations. Forty- eight hours later,the cell supernatant was collected and Collagen Ⅰ protein expression level was assessed. The LX- 2 cells were then co- cultured with IL- 32γ,and p38 MAPK inhibitors SB 203580 of different concentrations were added. Collagen Ⅰ RNA and protein expression levels were assessed by using real- time PCR and ELISA,respectively. Results IL- 32γ could induce Collagen Ⅰ expression in LX- 2cells. Moreover,Collagen Ⅰ expression was induced by IL- 32γ and recombinant Human Active p38α protein at dose-dependent manners. Collagen Ⅰ expression induced by IL- 32γ was inhibited by p38 MAPK. Conclusion IL- 32γ induces Collagen Ⅰ expression in LX- 2 cells via the activation of p38 MAPK signal pathway. IL- 32 might be involved in the pathogenesis of liver fibrosis via the induction of Collagen Ⅰ expression in LX- 2 cells.

关 键 词：白细胞介素-32 P38MAPK LX-2细胞 Ⅰ型胶原 

分 类 号：R733.71[医药卫生—肿瘤]