星形细胞上调基因1、β-连环素及周期素D1在肝细胞癌中的表达及临床意义  

Expression and clinical significance of astrocyte elevated gene-1,β-catenin,and cyclin D1 in hepatocellular carcinoma

作　　者：于营[1,2] 罗新华[3] 程明亮[1] 程云娟 

机构地区：[1]贵州医科大学感染病学教研室,贵阳550004 [2]秦皇岛市第一医院感染科,河北秦皇岛066000 [3]贵州省人民医院感染科,贵阳550002

出　　处：《临床肝胆病杂志》2016年第8期1549-1552,共4页Journal of Clinical Hepatology

摘　　要：目的探讨肝细胞癌(HCC)组织中星形细胞上调基因(AEG)1、β-连环素(β-catenin)及周期素D1(Cyclin D1)的表达及临床意义。方法随机选取2013年7月-2014年12月贵州省人民医院经手术及病理证实为HCC的癌组织标本和对应癌旁组织各40例,另选取8例正常肝组织作为对照组。采用免疫组化SP法检测AEG-1、β-catenin及Cyclin D1蛋白在HCC组织、对应癌旁肝组织及正常肝组织中的表达情况,并分析其表达与HCC临床病理因素的相关性。计数资料组间比较采用χ2检验或Fisher确切概率法,AEG-1、β-catenin、Cyclin D1在HCC中的相关性采用Spearman等级相关分析。结果 AEG-1、β-catenin及Cyclin D1在HCC组织及癌旁肝组织中的表达均高于正常肝组织,差异均有统计学意义(χ2值分别为7.840、4.274、8.817、4.274、9.919、4.850,P值分别为0.005、0.039、0.003、0.039、0.002、0.028)。AEG-1、β-catenin、Cyclin D1的阳性表达在性别、年龄、HBs Ag、肿瘤大小方面差异无统计学意义(P值均>0.05),而在病理分化程度、肝癌TNM分期及转移方面差异有统计学意义(P值均<0.05)。相关性分析显示,AEG-1蛋白的表达与β-catenin、Cyclin D1蛋白的表达呈正相关(r值分别为0.420、0.741,P值均<0.01)。结论 AEG-1、β-catenin及Cyclin D1在HCC的发生、发展过程中起着重要的作用;AEG-1可能通过上调Cyclin D1、β-catenin的表达和活性而促进HCC的发生和转移;联合检测三者的表达,可作为HCC基因治疗及预后评价的重要指标。Objective To investigate the expression and clinical significance of astrocyte elevated gene- 1（ AEG- 1）,β- catenin,and cyclin D1 in hepatocellular carcinoma（ HCC） tissues. Methods A total of 40 HCC samples and 40 samples of corresponding para- carcinoma tissues from the patients with pathologically confirmed HCC who underwent surgery in The People＇s Hospital of Guizhou from July 2013 to December 2014 were randomly selected,and 8 samples of normal liver tissues were selected as controls. The immunohistochemistry SP was used to measure the protein expression of AEG- 1,β- catenin,and cyclin D1 in HCC tissues,corresponding para- carcinoma tissues,and normal liver tissues,and the correlation between their expression and HCC clinicopathological characteristics was analyzed. The chi- square test or Fisher＇s exact test was used for comparison of categorical data between groups,and the Spearman rank correlation was used to analyze the correlation of AEG- 1 with β- catenin,and cyclin D1 in HCC. Results HCC tissues and para- carcinoma tissues showed significantly higher protein expression of AEG- 1,β- catenin,and cyclin D1 than normal liver tissues（ χ2= 7. 840,4. 274,8. 817,4. 274,9. 919,and 4. 850,P = 0. 005,0. 039,0. 003,0. 039,0. 002,and 0. 028）. The positive expression of AEG- 1,β- catenin,and cyclin D1 showed no significant differences across the patients with different sexes,ages,HBs Ag status,or tumor sizes（ all P〈0. 05）,but showed significant differences across the patients with different degrees of pathological differentiation,TNM stages for liver cancer,and metastases（ all P〈0. 05）. The correlation analysis showed that the protein expression of AEG- 1 was positively correlated with that of β- catenin and cyclin D1（ r = 0. 420 and 0. 741,both P〈0. 01）. Conclusion AEG- 1,β- catenin,and cyclin D1 may play vital roles in the development and progression of HCC. AEG- 1 may up- regulate the expression and activity of cyclin D1 and β- catenin and thus promote the development and

关 键 词：癌 肝细胞 星形细胞上调基因1 Β连环素 细胞周期蛋白D1 

分 类 号：R735.7[医药卫生—肿瘤]