BMSC对经基因沉默肝癌组织的影响  

Effect of BMSC on hepatocellular carcinoma tissues by gene interference

作　　者：李天然[1] 黄晓斌 宋斌 卢光明[3] 李延军[3] 

机构地区：[1]解放军第304医院放射科,北京100048 [2]解放军第九五医院放射科 [3]解放军南京总医院医学影像科

出　　处：《胃肠病学和肝病学杂志》2016年第8期860-864,共5页Chinese Journal of Gastroenterology and Hepatology

基　　金：国家自然科学基金资助项目(81271607);南京军区医药卫生重点项目资助(11Z035);国家博士后基金(2015M572810)

摘　　要：目的观察BMSC对经基因改造的具有不同转移潜能肝癌的影响。方法 63只裸鼠随机分成8组：MHCC97-L组（9只）、MHCC97-L NC组（8只）、MHCC97-L OPN沉默组（8只）、MHCC97-L TGFβ1沉默组（5只）、MHCC97-H组（8只）、MHCC97-H NC组（8只）、MHCC97-H OPN沉默组（9只）、MHCC97-H TGFβ1沉默组（8只）。肿瘤体积50 mm3左右开始瘤内注射人间充质干细胞BMSC,1×10^5/只,每周2次,连续4周。计算瘤体积抑制率（%）和瘤重抑制率（%）,BMSC对经基因沉默的肝癌动物模型干预结果进行病理检测。结果与MHCC97-L组比,BMSC对MHCC97-L NC组瘤体积和瘤重均有促进作用,与MHCC97-L组比,BMSC对MHCC97-L OPN沉默组瘤体积和瘤重均有明显的抑制作用,而BMSC对MHCC97-L TGFβ1沉默组瘤体积和瘤重均有促进作用。与MHCC97-H组比,BMSC对MHCC97-H NC组瘤体积和瘤重均有轻度抑制作用,与MHCC97-H组比,BMSC对MHCC97-H OPN沉默组和MHCC97-H TGFβ1沉默组瘤体积和瘤重均有显著的抑制作用。与MHCC97-H组及MHCC97-H NC组比较,MHCC97-H OPN沉默组纤维增生及核分裂象较少,而MHCC97-H TGFβ1沉默组纤维增生明显,核分裂象较多。与MHCC97-L比较,MHCC97-L NC、MHCC97-L OPN沉默组、MHCC97-L TGFβ1沉默组见较多坏死区、较多肿瘤细胞凋亡、坏死,并可见数个核分裂象。结论抑制肿瘤OPN表达BMSC可更好地抑制肿瘤的增殖,而抑制肿瘤TGFβ1表达BMSC并不能抑制肿瘤的增殖。相应的抗体沉默OPN可能是治疗肿瘤的一个比较好的靶点,配合BMSC生物治疗会提高治疗的生物效能。Objective To observe the effect of BMSC on hepatocellular carcinoma（ HCC） with different metastatic potential by gene interference. Methods Sixty-three nude mice were randomly divided into eight groups,including MHCC97-L blank control group（ 9 nude mice）,MHCC97-L gene negative control group（ 8 nude mice）,MHCC97-L OPN gene interference group（ 8 nude mice）,MHCC97-L TGFβ1 gene interference group（ 5 nude mice） and MHCC97-H blank control group（ 8 nude mice）,MHCC97-H gene negative control group（ 8 nude mice）,MHCC97-H OPN gene interference group（ 9 nude mice）,MHCC97-L TGFβ1 gene interference group（ 8 nude mice）. When tumor volume grown to 50 mm3,human mesenchymal stem cells（ BMSC） was injected,and 1 × 10^5/ one,two times a week,for 4weeks. Tumor volume inhibition rate（ %） and tumor weight inhibition rate（ %） were calculated,and pathological test was used for HCC animal model with gene silencing. Results Compared with the MHCC97-L blank control group,BMSC could promote the tumor volume and tumor weight of MHCC97-L NC group,and could inhibit tumor volume and tumor weight of MHCC97-L OPN gene interference group. However,BMSC could promote the tumor volume and tumor weight of MHCC97-L TGFβ1 gene interference group. Compared with the MHCC97-H blank control group,BMSC could mildly inhibit the tumor and tumor weight of MHCC97-H gene negative control group,and could inhibit tumor volume and tumor weight of MHCC97-H OPN and TGFβ1 gene interference groups. Compared with MHCC97-H blank control group and MHCC97-H gene negative control group,the fiber proliferation and nuclear division of MHCC97-H OPN gene interference group were less,while the MHCC97-H TGFβ1 gene interference group had obvious fiber hyperplasia and more nuclear division. Compared with MHCC97-L blank control group,MHCC97-L gene negative control group,MHCC97-L OPN gene interference group and MHCC97-L TGFβ1 gene interference group could see more necrotic areas,more tumor cell apoptosis,necrosis,and can be seen a n

关 键 词：肝癌 骨髓间充质干细胞 转移 骨桥蛋白 肿瘤生长转化因子 

分 类 号：R735.7[医药卫生—肿瘤]