维生素B_1与氯酚红的分子吸收光谱及应用  

Absorption Spectral Study of the Interaction Between Vitamin B_1 with Chlorophenol Red

作　　者：江蔓 杨智[1] 王凤怡 江虹[1] 

机构地区：[1]长江师范学院化学化工学院,重庆408100

出　　处：《分析科学学报》2016年第4期582-584,共3页Journal of Analytical Science

基　　金：重庆市教委科技基金(No:KJ1401226);长江师范学院科技基金(No:2014CXX0185)

摘　　要：建立了简便、快速测定维生素B1（VB1）的分光光度法。在弱酸性条件下,适量的VB1与氯酚红（CHR）反应生成具有明显正、负吸收峰的红色离子缔合物,最大正、负吸收波长分别为428nm、572nm,表观摩尔吸光系数（ε）分别为2.33×10^4 L/（mol·cm）（正吸收）和6.79×10^4 L/（mol·cm）（负吸收）。VB1的浓度在0～6.0mg/L范围内遵从比尔定律。该方法简便、快速、有较高的准确度和灵敏度,可用于市售奶粉中VB1的测定。The chromogenic reaction between vitamin B1 and chlorophenol spectrophotometry. A simple and rapid method for determination of vitamin Under weak-acid condition, an appropriate amount of vitamin B1 was reacte form a red ion association complexes with obvious positive absorption pe red was investigated by was therefore developed. d with chlorophenol red, to ak and negative absorption peak. The maximum positive and negative absorption wavelength was at 428 nm and 572 nm, respectively. The apparent molar absorptivity（e） was 2.33 ×10^4 L/（mol. cm） （positive absorption） and 6.79 ×10^4 L/（mol·cm） （negative absorption） ,respectively. Beer＇s law was obeyed in the range of 0-6.0 mg/L for vitamin B1. The method was applied to determine the content of vitamin B1 in market milk powder with the recovery andRSD（n=6） in the ranges of 98. 4%-101% and 1.5% 1.8% ,respectively.

关 键 词：维生素B1 氯酚红 奶粉 分子吸收光谱 

分 类 号：O657.32[理学—分析化学]