肝细胞癌中ChREBP基因CpG岛甲基化与mRNA表达的相关性  

Correlation of ChREBP mRNA Expression and Its CpG Island Methylation in Human Hepatocellular Carcinoma

作　　者：冉凤鸣[1] 罗成刚[2] 邱雪平[3] 黄一芳[3] 郑芳[3] 魏少忠[4] 

机构地区：[1]湖北省肿瘤医院肿瘤内科,武汉430079 [2]湖北省肿瘤医院放射科,武汉430079 [3]武汉大学中南医院基因诊断中心,武汉430071 [4]湖北省肿瘤医院胃肠外科,武汉430079

出　　处：《肿瘤防治研究》2016年第8期694-698,共5页Cancer Research on Prevention and Treatment

基　　金：国家重点基础研究发展计划(973计划)(2012CB720605)

摘　　要：目的检测肝细胞癌中Ch REBP基因Cp G岛甲基化水平及m RNA表达水平,并探讨两者的相关性。方法收集肝细胞癌及癌旁冰冻组织90例,采用亚硫酸氢钠处理结合克隆测序检测Ch REBP基因Cp G岛内29个Cp G位点的甲基化水平;采用荧光定量PCR检测其中31对新鲜冰冻组织中Ch REBP基因m RNA表达水平,分析甲基化水平与m RNA表达之间的关系。结果 Ch REBP基因的5、6、7、14号Cp G位点甲基化水平在肝细胞癌中显著低于其配对的癌旁组织(P<0.05)。其余Cp G位点及整体甲基化在肝细胞癌与癌旁组织中差异无统计学意义(均P>0.05)。15、18、20、23、26、29号Cp G位点在≥50岁年龄组的甲基化水平均高于<50岁年龄组(均P<0.05)。肝细胞癌中Ch REBP基因m RNA表达水平低于癌旁组织(P=0.003),但与甲基化无显著相关性(P>0.05)。结论肝细胞癌中Ch REBP基因m RNA表达水平低于癌旁组织,但这一改变可能不是通过影响DNA的甲基化水平实现的。Objective To detect the expression of Ch REBP m RNA and its Cp G island methylation in human hepatocellular carcinoma tissues and investigate their correlation. Methods Bisulfite Genomic sequencing was used to analyze the methylation level of 29 Cp G sites in Cp G island of Ch REBP in 45 paired hepatocellular carcinoma and adjacent non-tumor tissues. The expression of Ch REBP m RNA was detected in 31 paired hepatocellular carcinoma and adjacent non-tumor tissues using quantitative real-time PCR, meanwhile, the relationship between the level of methylation status and m RNA expression was analyzed. Results The methylation level of some Cp G sites（5, 6, 7, 14） were significantly lower in hepatocellular carcinoma tissues than those in the adjacent non-tumor tissues（P〈0.05）. There was no statistical significancet difference in the methylation level of other Cp G sites between hepatocellular carcinoma tissues and adjacent non-tumor tissues（all P〈0.05）. The methylation level of Ch REBP at Cp G 15, 18, 20, 23, 26 and 29 tended to be higher in patients with older age（≥50 years） than those in younger age（50 years）（all P〈0.05）. The m RNA expression of Ch REBP in hepatocellular carcinoma tissues was lower than that in adjacent non-tumor tissues（P=0.003）, however, no significant relationship was observed between DNA methylation and the m RNA expression of Ch REBP in hepatocellular carcinoma tissues（P〉0.05）. Conclusion There was a down-regulated expression of Ch REBP in hepatocellular carcinoma tissues, which could not be mediated by DNA methylation.

关 键 词：肝细胞癌 DNA甲基化 CHREBP MRNA表达 

分 类 号：R361.3[医药卫生—病理学]