鞘内β-七叶皂甙钠对神经病理性疼痛大鼠脊髓背角细胞因子的影响  

Effects of intrathecal β-Aescin sodium on cytokines in spinal dorsal horn of rats with neuropathic pain

作　　者：王保[1] 尧新华[1] 肖珍科[1] 鲁义[1] 周朴[1] 卿朝辉[1] 谭花[1] 

机构地区：[1]广州市中医医院麻醉科,广东广州510130

出　　处：《广州医科大学学报》2016年第2期6-8,共3页Academic Journal of Guangzhou Medical University

基　　金：广州市医药卫生科技项目(20131A 011034)

摘　　要：目的：观察鞘内β-七叶皂甙钠对神经病理性疼痛大鼠脊髓背角细胞因子的影响。方法：于健康雄性SD大鼠（体质量180～200 g）鞘内留置PE-10导管,置管1周后制作神经病理性疼痛模型。将18只神经病理性疼痛大鼠随机分成3组（每组6只）：生理盐水组、β-七叶皂甙钠20μg组和β-七叶皂甙钠40μg组。测定3组鞘内给药前、给药后20、40、60 min大鼠机械性阈值,计算最大效应百分比（ MPE）。采用RT-PCR法检测脊髓背角内TNF-a、IL-1β、IL-6的mRNA水平。结果：β-七叶皂甙钠20μg组、40μg组MPE均明显高于生理盐水组（P〈0.05）,TNF-a、IL-1β、IL-6 mRNA表达水平则明显低于生理盐水组（P〈0.05）。结论：鞘内注射β-七叶皂甙钠可产生抗神经病理性疼痛作用,其作用可能与其抑制脊髓背角内TNF-a、IL-1β、IL-6的mRNA表达有关。Objective：To investigate the effects of intrathecalβ-Aescin sodium on cytokines in spinal dorsal horn of rats with neuropathic pain. Methods：Healthy male SD rats （ body weight 180～ 200 g ） received intrathecal indwelling of PE-10 catheters. One week later,model of neuropathic pain was established in these rats by spinal nerve ligation （ SNL ） . Eighteen SNL rats were randomly selected and divided into 3 groups （ n=6 each）：normal saline group,20 μg β-Aescin sodium group and 40 μg β-Aescin sodium group. Before intrathecal administration and at 20,40 and 60 min after administration,we determined the mechanical threshold of rats,and thereby calculated the maximum percent effect （ MPE） . RT-PCR was used to detected the mRNA levels of TNF-a,IL-1βand IL-6 in the dorsal horn of spinal cord. Results：Compared with normal saline group,20μg and 40μgβ-Aescin groups showed significantly higher MPE values （P〈0.05）,and significantly lower mRNA expression levels of TNF-a,IL-1β and IL-6 （ P〈0. 05 ） . Conclusion：Intrathecal injection of β-Aescin may result in anti-neuropathic pain effect,which may be related to inhibition of TNF-a, IL-1β, and IL-6 mRNA expression in the dorsal horn of spinal cord.

关 键 词：Β-七叶皂甙钠 神经病理性疼痛 脊髓背角 细胞因子 

分 类 号：R575.2[医药卫生—消化系统]