维生素D受体基因FokI多态性与维吾尔族胃癌的相关性  

Correlation of gastric cancer and effects of vitamin D receptor gene Fok I polymorphism on C- reactive protein and D-dimer in Uygur nationality patients

作　　者：方法[1] 高洁[2] 韩峰[3] 王海江[1] 

机构地区：[1]新疆医科大学附属肿瘤医院胃肠外科,乌鲁木齐830011 [2]新疆医科大学附属肿瘤医院体检科,乌鲁木齐830011 [3]新疆医科大学附属肿瘤医院急诊科,乌鲁木齐830011

出　　处：《中华医学杂志》2016年第32期2541-2544,共4页National Medical Journal of China

基　　金：新疆医科大学创新基金（XJC2013105）

摘　　要：目的探讨维生素D受体基因FokI多态性作用于C-反应蛋白、D-二聚体对维吾尔族胃癌患者的影响。方法使用抽签法随机选取新疆肿瘤医院就诊的维吾尔族胃癌患者147例（A组）及同民族健康对照者151例（B组），采用多聚酶链反应一限制性酶切片段长度多态性法（PCR—RFLP）测定两组FokI位点多态性找寻胃癌易感基因；全部胃癌患者经PCR—RFLP测定按是否带有f基因分为FF组（a组）与Ff＋ff组（b组），比较组间C-反应蛋白及D-二聚体表达水平。结果胃癌患者的f等位基因表达频率高于健康对照组（57．8％与47．4％，P〈0．05）；含f等位基因（Ff＋ff）的研究对象患胃癌的风险较高（OR=2．85）；带有f等位基因组中C-反应蛋白、D-二聚体测定水平分别高于不含f等位基因组（4．72±1．65）与（2．81±0．93）、（2．79±1．21）与（1．09±0．65），两组结果比较差异均有统计学意义（均P〈0．05）。结论维生素D受体基因FokI酶切位点多态性与维吾尔族胃癌易感性有关，f等位基因可能是维吾尔族胃癌发生的危险因素之一。f等位基因可能是分别调节C-反应蛋白及D-二聚体水平的因素之一，与维吾尔族胃癌的发生有关。Objective This study aimed to determine the correlation of Uygur gastric cancer and the polymorphism effects of vitamin D receptor gene Fok I on C-reactive protein and D-dimer. Methods Method of random sampling was used to select 147 Uygur patients with gastric cancer who admitted at Tumor Hospital of Xinjing Medical University （ Group A） and 151 healthy controls with the same nationality （ Group B）. Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） method was used to determine Fok Ⅰ loci polymorphism in both groups to identify the gastric cancer susceptibility gene. All patients were divided into Group FF （ Group a） and Group Ff ＋ ff （ Group b） by PCR-RFLP according to the f-gene carrier to compare the expression level of C-reactive protein and D-dimer. Results The expression frequency of the f allelic gene of patients with gastric cancer was higher than that of healthy control patients （57.8 % vs 47.4% , P 〈 0. 05 ）. The carriers of the f allelic gene （ Ff ＋ if） had increased risk of gastric cancer （ OR = 2. 85 ）. Measurement levels of C-reactive protein and D-dimer in the f allelic gene group were higher than those of the non-f allelic gene group （4. 72 ± 1.65 vs 2. 81 ±0. 93, and 2. 79 ± 1.21 vs 1.09 ± 0. 65, respectively）, and the differences were statistically significant （P 〈 0. 05 ）. Conclusions The Fok I loci polymorphism of vitamin D receptor gene was related to gastric cancer susceptibility in Uygur. f allelic gene may be one of the risk factors of gastric cancer in Uygur. Moreover, f allelie gene may be a factor that increased the level of C-reactive protein and D-dimer in Uygur patients with gastric cancer.

关 键 词：维生素D受体 胃肿瘤 C-反应蛋白 D-二聚体 f等位基因 

分 类 号：R735.2[医药卫生—肿瘤]