螺旋藻多糖对阿尔茨海默病小鼠脑线粒体呼吸链酶活性的影响  

Effect of spirulina polysaccharide on activity of mitochondrial respiratory chain enzyme in Alzheimer's disease model mice

作　　者：林智君[1] 陈煜森[1] 钟望涛[1] 尹小健[1] 刘洲[1] 贾真[1] 赵斌[2] 

机构地区：[1]广东医学院附属医院神经内科,广东湛江524023 [2]广东医学院附属医院神经病学研究所,广东湛江524023

出　　处：《中华脑科疾病与康复杂志（电子版）》2016年第2期79-82,共4页Chinese Journal of Brain Diseases and Rehabilitation（Electronic Edition）

基　　金：国家自然科学基金(青年科学基金项目)(81400986);广东省自然科学基金(2014A030313541);湛江市科技攻关计划项目(2014B01201)

摘　　要：目的从脑线粒体呼吸链酶活性角度探讨螺旋藻多糖对阿尔茨海默病(AD)小鼠脑线粒体损伤的保护作用。方法将24只小鼠随机分为3组,正常组、模型组以及实验组,模型组、实验组小鼠腹腔均注射D-半乳糖120 mg/kg和亚硝酸钠100 mg/kg制备AD模型,正常组注射同等剂量的生理盐水。同时给予实验组灌胃螺旋藻多糖67.0 mg·kg^(-1)·d^(-1),连续8周,在给药第8周后,进行Morris水迷宫实验和生化指标测试。结果与正常组相比,模型组、实验组120 s内跨越平台的次数(3.9±1.90,3.8±1.41 vs.5.9±0.47)明显减少(P<0.05),在目标象限的探索时间[(26.93±5.20)s,(27.25±5.79)s vs.(9.54±1.21)s]均明显增加(P<0.05);与模型组[(57.00±6.89)pg/g]相比,正常组[(42.75±5.23)pg/g]、实验组[(48.14±8.13)pg/g]小鼠脑部β淀粉样蛋白(Aβ)含量均减少(P<0.05),脑线粒体呼吸链酶活性结果显示,D-半乳糖联合亚硝酸钠诱导AD可降低复合物(CⅠ,CⅢ,CⅣ)活性,螺旋藻多糖可抑制复合物(CⅢ,CⅣ)减少(P<0.05)。结论螺旋藻多糖可影响AD模型小鼠脑线粒体呼吸链酶活性,可能使ATP的合成增加。Objective To study spirulina polysaccharide on mitochondrial of Alzheimer＇s disease （AD） model mice from the viewpoint of respiratory chain enzyme activity. Methods Mice were injected D- galactose 120 mg/kg and sodium nitrite 100 mg/kg to establish AD model. 24 mice were randomly divided into 3 groups, the normal group, model group and experimental group, the experimental gastriced Spiruliua polysaccharide of 67.0 mg ·kg-1 d -1 for 8 weeks, Morris water maze experiment test and biochemical indicators were tested. Results Compared with the normal group, crossed platform frequency （3.9 ± 1.90, 3.8 ± 1.41 vs. 5.9 ± 0. 47 ） decrease Significantly group in the 120 s （ P 〈 0. 05 ）, the target quadrant exploration time [ （26. 93 ±5.2） s, （27.25 ±5.79） s vs. （9. 54 ± 1.21 ） s] were significantly increased both in model group and experimental group （ P 〈 0.05 ）. Compared with model group [ （ 57.00 ＋_ 6. 89 ） pg/g ], amyloid 13 content in brain of normal group [ （42. 75 ± 5.23 ） pg/g ] and experimental group [ （ 48. 14 ± 8. 13 ） pg/g] were reduced （ P 〈 0. 05 ） , cerebral mitochondrial respiratory chain enzymes showed joint D- galactose induced by sodium nitrite AD can reduce compounds （C I , CⅢ, CⅣ）activity, spiral alga inhibits the decrease of compounds （ C Ⅲ, C Ⅳ） （ P 〈 0. 05 ）. Conclusion Spirulina polysaccharide can affect activity of mitochondrial respiratory chain enzyme of AD model mice, and may increase the ATP synthesis.

关 键 词：阿尔茨海默病 螺旋藻属 线粒体呼吸链酶 

分 类 号：R749.16[医药卫生—神经病学与精神病学]