逆灸关元、命门穴对力竭运动大鼠糖原、乳酸的影响  

Preventive moxibustion at Guanyuan(CV-4) or mingmen(DU-4)on glycogen and lactic acid of exhaustive exercise rats

作　　者：李凯歌[1] 孙志芳[1] 王洋[1] 谭莉华[1] 莫捷[1] 张露芬[1] 任晓暄[1] 郭孟玮[1] 李晓泓[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《长春中医药大学学报》2016年第4期681-683,共3页Journal of Changchun University of Chinese Medicine

基　　金：国家自然科学基金课题(90209026);国家中医药管理局针灸特色疗法评价重点研究室基金

摘　　要：目的比较逆灸关元和命门穴对大鼠力竭运动时糖原、乳酸的影响,探讨逆灸不同经穴对调节机体能量代谢的作用。方法将大鼠按游泳能力随机分为:空白对照组、逆灸关元组、逆灸命门组、力竭对照组、逆灸关元+力竭组和逆灸命门+力竭组,逆灸组预先进行艾灸,干预20 d后进行力竭游泳,观察力竭时间并采用生化法检测肝糖原、肌糖原、肌乳酸、血乳酸的含量。结果与空白对照组相比,逆灸关元和命门组大鼠肝糖原、肌糖原、肌乳酸、血乳酸含量均无统计学意义(P>0.05),力竭对照组肝糖原、肌糖原含量下降(P<0.01),肌乳酸、血乳酸含量上升(P<0.01);与力竭对照组相比,逆灸关元+力竭组和逆灸命门+力竭组的力竭游泳时间延长(P<0.01);逆灸关元+力竭组肝糖原含量升高、肌糖原无统计学意义(P<0.01,P>0.05),肌乳酸、血乳酸含量下降(P<0.05,P<0.01),逆灸命门+力竭组肝糖原、肌糖原含量升高(P<0.01,P<0.05),肌乳酸、血乳酸含量下降(P<0.01),逆灸命门+力竭组的肌乳酸含量较逆灸关元+力竭组下降更明显(P<0.05)。结论逆灸关元和命门穴均可提高大鼠的运动耐力,使大鼠力竭时间延长,其作用可能与其加强能量代谢,提高糖原利用率并降低乳酸堆积有关,逆灸命门穴对肌乳酸的清除力度较关元穴更为明显。Objective Influence of compared moxibustion Guanyuan （CV-4） and Mingmen （DU-4） acupoint on rats of exercise glycogen,lactic acid.In order to explore the effect of pre-moxibustion at different acupoints on role.Methods Depend on swimming exhausted time,the rats were randomly divide into blank control group,Guanyuan pre-moxibustion group,Mingmen pre-moxibustion group,exhaustive control group,Guan yuan pre-moxibustion exhaustive group,Mingmen pre-moxibustion exhaustive group.Moxibustion group pre-moxibustion intervention after 20 days of exhaustive swimming.Observing the exhaustive swimming time and the biochemical method to detect liver glycogen,muscle glycogen,muscle lactic acid,blood lactic acid content.Results Compared with blank control group,pre-moxibustion group on Guanyuan and Mingmen rat liver glycogen and muscle glycogen,muscle lactate,blood lactic acid content were not different （P ＞ 0.05）,exhaustive control group of liver glycogen and muscle glycogen content was decreased （P ＜ 0.01）,muscle lactate and blood lactic acid content increased （P ＜ 0.01）;Compared with exhaustive control group,pre-moxibustion group on Guanyuan and Mingmen swimming time was prolonged （P ＜ 0.01）;Guan yuan pre-moxibustion exhaustive group of hepatic glycogen increased and muscle glycogen had no difference （P ＜0.01,P ＞0.05）,muscle lactate and blood lactic acid decreased （P ＜ 0.05,P ＜0.01）,Mingmen pre-moxibustion exhaustive group of liver and muscle glycogen increased （P ＜ 0.01,P ＜ 0.05）,muscle lactate,blood lactic acid decreased（P ＜0.01,P ＜0.01）,Compared Mingmen pre-moxibustion exhaustive group with Guan yuan pre-moxibustion exhaustive group muscle lactic acid decreased （P ＜ 0.05）.Conclusion Pre-moxibustion Guanyuan and Mingmen can improve rats exercise tolerance and prolong its exhaustive time,it may be related to strengthen improve the energy metabolism of glycogen utilization rate and reduce lactic acid accumulation,inverse moxibustion on Mingmen of muscle lact

关 键 词：逆灸疗法 力竭运动 糖原 乳酸 

分 类 号：R285.5[医药卫生—中药学]