高效液相色谱法建立黄芪多糖特征图谱研究  

Establishing specific chromatogram of Milkvetch Root polysaccharides by HPLC

作　　者：杨献玲[1] 徐多多[2] 高阳[2] 李红月[2] 王海洋[2] 

机构地区：[1]吉林省药品检验所,长春130033 [2]长春中医药大学,长春130117

出　　处：《长春中医药大学学报》2016年第4期701-703,共3页Journal of Changchun University of Chinese Medicine

基　　金：中华人民共和国国家科技部"中药提取物标准研究平台建设"(2012ZX09304005004)

摘　　要：目的探讨高效液相色谱法建立黄芪多糖特征图谱。方法利用高效液相色谱法对PMP柱前衍生化的黄芪多糖进行糖组成分析。结果黄芪多糖特定图谱由鼠李糖、葡萄糖、半乳糖、阿拉伯糖4种单糖4个共有峰组成。结论高效液相色谱法建立的黄芪多糖特征图谱,其操作方法简便,具有良好的重复性及稳定性,可有效控制黄芪多糖的内在质量。Objective To establish specific chromatogram of Milkvetch Root polysaccharides.Methods The monosaccharide composition of Milkvetch Root polysaccharides was determined by HPLC after preparing pre-column derivatization as PMP derivant.Results There were 4 peaks from the specific chromatogram which were rhamnose,glucose,galactose and arabinose monosaccharide respectively.Conclusion The HPLC method establishing specific chromatogram is a simple,rapid,sensitive and convenient,which can be applied to the quality control of Milkvetch Root polysaccharides.

关 键 词：黄芪多糖 鼠李糖 单糖 图谱 高效液相色谱法 

分 类 号：R284.3[医药卫生—中药学]