GDNF剪接体α-pro-GDNF在大鼠C6胶质瘤细胞中的表达及其意义  

The expression and significaceof GDNF splice variant α-pro-GDNF in rat C6 glioma cells

作　　者：魏赫[1,2] 李亨[2] 雷宇[2] 于如同[3] 虞正权[1] 高殿帅[2,3] 

机构地区：[1]苏州大学附属第一人民医院神经外科,苏州215000 [2]徐州医学院解剖学和神经生物学教研室,徐州221000 [3]徐州医学院附属医院神经外科,徐州221000

出　　处：《神经解剖学杂志》2016年第4期471-476,共6页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(81372698);江苏省自然科学基金(BK20130212)

摘　　要：目的:探讨大鼠C6胶质瘤细胞中GDNF选择性剪接体的表达情况及意义。方法:通过检测C6胶质瘤细胞中GDNF基因△78位点剪接体α-pro-GDNF mRNA和β-pro-GDNF mRNA及其受体蛋白GFRα1、GFRα2、Ret和NCAM表达,结合介导肿瘤迁移相关蛋白RhoA、Cdc42、Rac1的表达情况,分析其相关关系。结果:(1)C6胶质瘤细胞中,可以检测到α-pro-GDNF和β-pro-GDNF的mRNA表达,且α-pro-GDNF mRNA表达水平比β-proGDNF高;与星形胶质细胞相比,α-pro-GDNF mRNA表达水平的增高趋势较β-pro-GDNF明显;(2)C6胶质瘤细胞中RhoA mRNA表达水平与α-pro-GDNF mRNA表达水平成显著正相关(R2=0.699,P<0.05),Cdc 42 mRNA表达水平与α-pro-GDNF mRNA表达水平呈显著正相关(R2=0.803,P<0.05),与β-pro-GDNF mRNA表达相关性不显著。结论:C6胶质瘤中α-pro-GDNF具有优先表达,可能与胶质瘤细胞的迁移具有相关性。Objective： To investigate the expression and significance of GDNF splice variants in rat C6 glioma cells.Methods： Real-time PCR techniques were used to detect the expression level of mRNA of both GDNF splice variants（ α-and β-pro-GDNF）,GDNF receptors（ GFRα1,GFRα2,Ret and NCAM）,associated proteins mediated tumor migration（ RhoA,Cdc42,Rac1） in normal rat astrocytes and C6 glioma cells,and the relationship between them.Results：（ 1） Both α- and β-pro-GDNF mRNA were expressed in C6 glioma cells,and α-pro-GDNF mRNA expression levels were higher than β-pro-GDNF; Compared to astrocytes,the elevated degree of expression levels of α-pro-GDNF mRNA was significantly more than β-pro-GDNF.（ 2） The mRNA expression level of RhoA（ R2= 0. 699,P〈0. 05） and Cdc42（ R2= 0. 803,P〈0. 05） had a significantly positive correlation with α-pro-GDNF mRNA expression level,but no relationship with β-pro-GDNF mRNA expression level. Conclusion： GDNF alternative splicing α-pro-GDNF expressed abuadautly in C6 glioma cells and was possibly correlated with the migration of glioma cells.

关 键 词：胶质瘤 GDNF 选择性剪接 RhoA CDC42 RAC1 

分 类 号：R739.41[医药卫生—肿瘤]