结直肠异常隐窝灶中BAI1、VEGF蛋白表达及意义  

Expression of BAI1 and VEGF in aberrant crypt foci of colorectum and their implications

作　　者：刘芳[1] 部晶 张鹏[3] 安亚伟[4] 尚雪飞[1] 彭燕[1] 成志勇[1] 

机构地区：[1]河北省保定市第一医院病理科,保定071000 [2]河北省保定市第一医院消化科,保定071000 [3]河北省保定市第一医院结直肠外科,保定071000 [4]河北省保定市第一医院普外科,保定071000

出　　处：《临床与实验病理学杂志》2016年第8期856-859,共4页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的观察BAI1、VEGF在结肠异型增生性异常隐窝灶(aberrant crypt foci,ACF)、管状腺瘤及腺瘤癌变中的表达。方法联合放大内镜窄带成像(narrow-band imaging,NBI)技术观察ACF,采用免疫组化法检测40例异型增生性ACF、40例结肠管状腺瘤伴异型增生、35例管状腺瘤癌变及32例结直肠正常黏膜标本中BAI1和VEGF的表达。结果 BAI1蛋白在结直肠正常黏膜、异型增生性ACF、结肠管状腺瘤伴异型增生及管状腺瘤癌变组中的阳性率逐步降低,分别为93.8%、72.5%、45%、11.4%,且异型增生性ACF组阳性率明显低于结直肠正常黏膜组(P<0.05),结肠管状腺瘤伴异型增生组阳性率明显低于异型增生性ACF组(P<0.05),管状腺瘤癌变组阳性率明显低于管状腺瘤伴异型增生组(P<0.05);VEGF蛋白在结直肠正常黏膜、异型增生性ACF、管状腺瘤伴异型增生及管状腺瘤癌变组中的阳性率逐步升高,分别为9.4%、52.5%、62.5%、94.3%,且异型增生性ACF组阳性率明显高于结直肠正常黏膜组(P<0.05),管状腺瘤癌变组阳性率明显高于异型增生性ACF组及管状腺瘤伴异型增生组(P<0.05);在异型增生性ACF、管状腺瘤及管状腺瘤癌变过程中BAI1和VEGF表达呈负相关(rs=-0.656,P<0.05)。结论 BAI1低表达与VEGF高表达可能在结直肠癌前病变中起一定作用,可能为结直肠肿瘤的早期诊治提供新的作用靶点。Purpose To observe expression of BAIl and VEGF in colonic dysplastic aberrant crypt foci （ aberrant crypt foci, ACF）, tubular adenomas and adenocarcinoma deriving from tubular adenomas. Methods ACF was observed using narrow-band imaging （NBI） technique. The expression levels of BAIl and VEGF in 40 cases of dysplastic ACF, 40 cases of sporadic colorectal tubular ade- noma with dysplasia （SCTA-D）, 35 cases of adenocarcinoma deriving from sporadic colorectal tubular adenoma （SCTA-Ca）, and 32 cases of normal control （NC） were studied using immunohistochemical staining. Results The positive expression rate of BAIl was decreasing from NC, dysplastic ACF, SCTA-D to SCTA-Ca with positive expression rate of 93.8%, 72. 5%, 45%, and 11.4%, respec- tively. The positive expression rate of BAIl in the dysplastic ACF group was significantly lower than that in the normal control group （P 〈 0. 05）. The positive expression rate of VEGF was increasing from NC, dysplastic ACF, SCTA-D to SCTA-Ca with the positive rate of 9.4%, 52. 5%, 62. 5% and 94. 3%, respectively. Positive expression rate in dysplasia ACF group was significantly higher than that in the normal control group （ P 〈 0. 05 ）, that was higher than that of dysplastic ACF group and adenoma tubular adenoma with dysplasia group （P 〈 0. 05）. In the process of ACF, dysplastic tubular adenoma and adenoma in BAIl was negatively correlated with VEGF expression （rs = - 0. 656, P 〈 0. 05 ）. Conclusion The abnormal expression of BAIl and VEGF may play a role in the pathogenesis of colorectal cancer. This study may provide a new target for the early diagnosis and treatment of colorectal cancer. imaging

关 键 词：结直肠肿瘤 结直肠管状腺瘤 异常隐窝灶 BAI1 VEGF 窄带成像技术 

分 类 号：R735.3[医药卫生—肿瘤]