Lipogenesis in myoblasts and its regulation of CTRP6 by AdipoR1/Erk/PPARγ signaling pathway  

作　　者：Wenjing Wu Yunmei Sun Chen Zhao Cunzhen Zhao Xiaochang Chen Guoqiang Wang Weijun Pang Gongshe Yang 

机构地区：[1]Laboratory of Animal Fat Deposition & Muscle Development, College of Animal Science and Technology,Northwest A&F University, Yangling 712100, China

出　　处：《Acta Biochimica et Biophysica Sinica》2016年第6期509-519,共11页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the National Key Basic Research Program of China （Nos. 2015CB943102 and 2012CB124705） and the National Natural Science Foundation of China （Nos. U1201213 and 31572366）.

摘　　要：The induced lipogenesis and its regulation in C2C12 myoblasts remain largely unclear. Here, we found that the cocktail method could significantly induce lipogenesis through regulating lipid metabolic genes and Erk1/2 phosphorylation in myoblasts. Meanwhile, the expression and secretion of CTRP6 were increased during ectopic lipogenesis. Moreover, CTRP6 knockdown downregulated the levels of lipogenic genes and phosphorylated Erk1/2 （p-Erk1/2） in the early lipogenic stage, whereas up-regulated p-Erk1/2 in the terminal differentiation. Interestingly, the effect of CTRP6 siRNA was attenuated by U0126 （a special p-Erk1/2 inhibitor） in myoblasts. Furthermore, AdipoR1, not AdipoR2, was first identified as a receptor of CTRP6 during the process of mitotic clonal expansion. Collectively, we suggest that CTRP6 mediates the ectopic lipogenesis through AdipoR1/ErldPPARγ signaling pathway in myoblasts, Our findings will shed light on the novel biological function of CTRP6 during myoblast lipogenesis and provide a hopeful direction of improving meat quality of domestic animal by lipogenic regulation in skeletal muscle myoblasts.The induced lipogenesis and its regulation in C2C12 myoblasts remain largely unclear. Here, we found that the cocktail method could significantly induce lipogenesis through regulating lipid metabolic genes and Erk1/2 phosphorylation in myoblasts. Meanwhile, the expression and secretion of CTRP6 were increased during ectopic lipogenesis. Moreover, CTRP6 knockdown downregulated the levels of lipogenic genes and phosphorylated Erk1/2 （p-Erk1/2） in the early lipogenic stage, whereas up-regulated p-Erk1/2 in the terminal differentiation. Interestingly, the effect of CTRP6 siRNA was attenuated by U0126 （a special p-Erk1/2 inhibitor） in myoblasts. Furthermore, AdipoR1, not AdipoR2, was first identified as a receptor of CTRP6 during the process of mitotic clonal expansion. Collectively, we suggest that CTRP6 mediates the ectopic lipogenesis through AdipoR1/ErldPPARγ signaling pathway in myoblasts, Our findings will shed light on the novel biological function of CTRP6 during myoblast lipogenesis and provide a hopeful direction of improving meat quality of domestic animal by lipogenic regulation in skeletal muscle myoblasts.

关 键 词：CTRP6 C2C12 myoblast LIPOGENESIS AdipoR1/Erk/PPARγ signaling pathway 

分 类 号：Q51[生物学—生物化学] Q257