响应曲面法优化黄花败酱总皂苷提取工艺及体外促进肠平滑肌收缩活性  

Optimization of extraction technology of total saponins from Patrinia scabiosaefolia by response surface method and its contraction effect on isolated rat intestinal smooth muscle

作　　者：崔文燕[1,2] 刘素香[3] 赵艳敏[1] 张铁军[3] 宋晓凯[2] 刘岱琳[1] 孙乃强[1] 郑雅婷[1] 

机构地区：[1]中国人民武装警察部队后勤学院,天津300162 [2]淮海工学院,江苏连云港222005 [3]天津药物研究院,天津300193

出　　处：《中草药》2016年第12期2078-2083,共6页Chinese Traditional and Herbal Drugs

摘　　要：目的响应曲面法优化黄花败酱总皂苷(TSPS)的提取工艺及研究TSPS促进大鼠离体肠平滑肌收缩活性。方法以齐墩果酸为对照品,采用香草醛.冰醋酸显色,利用紫外分光光度计在550 nm处测定TSPS的量。以TSPS提取率作为响应值,在单因素试验的基础上采用响应曲面法考察提取溶剂、料液比、提取时间及其交互作用对TSPS提取效果的影响。并且测定了优化提取工艺过程中获取的不同TSPS提取物的促肠平滑肌收缩活性,结合生物活性全面评价TSPS提取工艺。结果TSPS的最佳提取工艺条件为提取溶剂75%乙醇,料液比1:12,提取时间为1.5 h,提取温度90℃、提取2次。在此工艺条件下TSPS的提取率达到10.96 mg/g,其对大鼠离体肠平滑肌的收缩作用强弱表现为结肠>回肠>空肠>十二指肠,质量浓度达0.32 mg/mL时促进离体肠收缩效果最佳。结论经优化后的TSPS提取工艺条件具有提取率高,提取工艺高效、稳定等特点,且TSPS具有显著的促进大鼠离体肠平滑肌收缩功能,其功效与提取率呈正相关。Objective To optimize the extraction technology for total saponins from Patrinia scabiosaefolia （TSPS） by response surface method. At the same time, the function of promoting intestinal peristalsis by total saponins was investigated. Methods UV spectrophotometric method was used to determine the contents of total saponins in P. scabiosaefolia at the wavelength of 510 nm by taking oleanolic acid as standard substance, and vanillin acetic acid as chromogenic reagent. The extraction rate of total saponins was used as index to evaluate the technology based on single factor test, in which three factors were considered the concentration of extraction solvent, solid-liquid ratio, extraction time, and their interaction on extraction were studied by response surface method. The contraction effect and signal changes of total saponins extract of P scabiosaefolia on intestinal smooth muscle were recorded by tension transducer to the BL-420 biological experimental multi-channel physiological signal acquisition and processing system. Results The best extraction conditions were as follows： ethanol concentration of 75% alcohol, solid-liquid ratio of 1：12, extraction time for 1.5 h, twice and extraction temperature of 90 ~C. The optimized extraction rate could reach 10.96 mg/g. P. scabiosaefolia extracts obtained using the optimized method showed significantly contraction effect on rat intestinal smooth muscle and the results were displayed as： colon 〉 colon 〉 jejunum 〉 duodenum. The concentration at 0.32 mg/mL has the best effect of gastrointestinal contraction. Conclusion The optimized extraction technology is stable and effective, and with higher extraction rate. The extract of P scabiosaefolia has a significant promotion effect on intestinal smooth muscle of rat, which shows positive correlation with the rate of extract.

关 键 词：响应曲面分析法 黄花败酱 总皂苷 提取工艺 离体肠平滑肌收缩 

分 类 号：R284.2[医药卫生—中药学]