芪白平肺颗粒醇提部位化学成分的UPLC-Q-TOF-MS鉴定  

UPLC-Q-TOF-MS analysis on constituents from ethanol extract fraction herbs of Qibai Pingfei Granule

作　　者：商晶[1,2] 靳瑞婷 李雪峰[1,2] 秦建平[1,2] 吴云[1,2] 丁岗[1,2] 王振中[1,2] 萧伟[1,2] 

机构地区：[1]江苏康缘药业股份有限公司,江苏连云港222047 [2]中药制药过程新技术国家重点实验室,江苏连云港222047

出　　处：《中草药》2016年第13期2246-2251,共6页Chinese Traditional and Herbal Drugs

基　　金：科技部重大新药创制现代中药创新集群与数字制药技术平台(2013ZX09402203)

摘　　要：目的研究芪白平肺颗粒醇提部位化学成分及醇提部位药材的合提与分提的差异性。方法以UPLC-Q-TOF-MS作为分析方法,通过对比各色谱峰的保留时间、质谱图特征,结合文献报道,确认芪白平肺颗粒中醇提部位的化学成分,并以主成分分析明确醇提部位药材分提与合提的差异性。结果对芪白平肺颗粒醇提部位中产生的26个离子进行鉴定,确定了22个离子的化学结构式,并推测了另外4个离子的化学结构式。并通过主成分分析对人参黄芪分提提取液和合提提取液进行分析,确定了12个化合物为人参黄芪合提与分提的差异物。结论本实验为完善芪白平肺颗粒的工艺和质量标准研究提供参考依据。Objective To study the chemical constituents from the ethanol extract fraction herbs of Qibai Pingfei Granule（QPG）, the difference of mixed decoction and separated decoction of ethanol extract fraction herbs in QPG. Methods UPLC-Q-TOF-MS was used as the analytic method. The information on quasi-molecular ion, fragment ions, retention time, and references were synthetically analyzed to confirm the constituents in the ethanol extract fraction herbs of QPG. Principal components analysis was applied to process the acquired data, and marked differences between mixed decoction and separated decoction in the ethanol extract fraction herbs of QPG. Results Twenty-two compounds were identified, and four compounds were tentatively identified among 26 ions. In addition, twelve compounds were determined as markers to distinguish the mixed decoction from the separated decoction of the ethanol extract fraction herbs in QPG. Conclusion The findings obtained from the study can provide the useful information for the determination of bioactive substances, the perfection of quality standard, and the preparation technology of QPG.

关 键 词：芪白平肺颗粒 UPLC-Q-TOF-MS 主成分分析 分提 合提 

分 类 号：R284.1[医药卫生—中药学]