骨髓间充质干细胞对癫痫模型认知及海马区GABAA受体表达的影响  

Effect of bone marrow mesenchymal stem cells on cognition and the expression of GABAARs in the hippocampus in a rat model of epilepsy

作　　者：黑悦 刘卫平[1] 魏礼洲[1] 伊西才[1] 陈晓燕[1] 

机构地区：[1]第四军医大学西京医院神经外科,陕西西安710032

出　　处：《中华神经外科疾病研究杂志》2016年第4期293-296,共4页Chinese Journal of Neurosurgical Disease Research

基　　金：陕西省科技统筹基金资助项目(2013KTCL03-08);社会发展引导计划-医学研究基金资助项目(SF1423-2)

摘　　要：目的探究骨髓间充质干细胞(BMSCs)移植对大鼠癫痫模型认知功能及海马CA1区域γ氨基丁酸A受体(GABAARs)的表达的影响。方法成年雄性SD大鼠30只随机分为正常组(n=10),模型组(n=10)和实验组(n=10)。采用匹鲁卡品诱导癫痫模型,2 h后移植进行BMSCs移植,28 d后水迷宫检测认知变化,免疫组化和实时荧光定量PCR(RT-PCR)检测大鼠海马CA1区GABAARs的表达变化。结果 RT-PCR和免疫组化结果显示,模型组相比正常组GABAARα1表达水平(mRNA以及免疫组化染色强度)下调(P<0.05)而GABAARα4上调(P<0.05),移植组GABAARs相关改变比模型组轻微(P<0.05),且认知功能改善明显(P<0.05)。结论 BMSCs移植可调节大鼠癫痫模型中海马区域GABAARs表达水平并减轻癫痫相关认知障碍。Objective The effect of bone marrow mesenchymal stem cells( BMSCs) transplantation on epilepsy-induced cognitive deficits and the altered expression of GABAA receptors( GABAARs) in the hippocampal CA1 subfield is discussed. Methods A total of 30 adult male SD rats were randomly divided into negative control group( n =10),positive control group( n = 10) and the experimental group( n = 10). BMSCs transplantation was performed at 2 h after induction of SE. After 28 days,morris water maze( MWM) test was performed to detect the cognitive changes,and immunohistochemistry and real-time polymerase chain reaction( RT-PCR) were used to evaluate the expression of GABAARα1 and α4 subunits in the hippocampal CA1 subfield. Results RT-PCR and immunohistochemistry showed that compared to normal group, GABAAR α1 expression( mRNA and immunohistochemical staining intensity) was decreased( P < 0. 05) while the expression of GABAAR α4 was increased( P <0. 05) in the positive control group; BMSCs transplants partly reversed these changes( P <0. 05),and MWM showed that cognitive ability in the experimental group was improved significantly( P < 0. 05) compared to positive control group. Conclusion BMSCs transplantation can regulate the expression of GABAARs in the hippocampus and restore the cognitive ability in a rat model of epilepsy.

关 键 词：骨髓间充质干细胞 癫痫 认知障碍 细胞移植 GABAA型受体 

分 类 号：R742.1[医药卫生—神经病学与精神病学]