YKL-40对MGMT高表达的人胶质瘤干细胞“干性”的影响  

Effect of YKL-40 on the "stemness" of human glioma stem cells with high expression of MGMT

作　　者：陈蔚军[1] 章薇[1] 章翔[1] 

机构地区：[1]第四军医大学西京医院神经外科,陕西西安710032

出　　处：《中华神经外科疾病研究杂志》2016年第4期309-312,共4页Chinese Journal of Neurosurgical Disease Research

基　　金：国家自然科学基金资助项目(81302174)

摘　　要：目的研究YKL-40在MGMT高表达的人胶质瘤干细胞(h GSCs)中对其干性的影响。方法收集临床胶质母细胞瘤样本,行分离、培养并进一步鉴定为h GSCs。YKL-40慢病毒干涉后,采用单细胞克隆球形成实验、Brd U细胞增值实验检测、RT-PCR法和Western blot法检测h GSCs中干性表达及生物学功能的改变。结果所入围的h GSCs中MGMT均为高表达,并且高表达YKL-40的h GSCs其单细胞成球能力、细胞增殖能力及干性表达均高于低表达YKL-40的h GSCs(P<0.05)。结论YKL-40在h GSCs干性维持的过程扮演了重要的角色,YKL-40有可能作为GBM手术后分子靶向治疗的关键靶点,并作为临床GBM诊治提供新的思路。Objective The effect of YKL-40 on the stemness of human glioma stem cells （hGSCs） with high expression of MGMT was investigated. Methods The hGSCs were isolated and identified from clinical glioblastoma specimen. After con_finning the transduction effect of hGSCs with YKL-40 lentivirus, single cell sphere formation assay, cell proliferation assay, RT-PCR and Western blot analysis were used to detect the expression of ＂stenmess＂ markers and the changes of biological function in hGSCs. Results MGMT protein was highly expressed in all the selected hGSCs. Single cell formation, cell proliferation and ＂stemness＂ of hGSCs with highly expressed YKL-40 were better than those of hGSCs with lower expression of YKL-40. Conclusion YKL-40 plays an important role in the process of ＂stemness＂ maintenance, and it might be the key target for the molecular therapy after clinical surgery, however, it can also provide new clinical ideas for the diagnosis and treatment of glioblastoma multiforme.

关 键 词：胶质母细胞瘤 肿瘤干细胞 YKL-40 MGMT 

分 类 号：R739[医药卫生—肿瘤]