琥珀酸对幼龄大鼠小脑谷氨酸能突触传递的抑制作用  

Inhibition of succinic acid on cerebellar glutamatergic synaptic transmission in neonatal rats

作　　者：何海燕[1,2] 陈静[3] 晋芙丽 李凌[1] 杜永平[3] 张月萍[1] 

机构地区：[1]第四军医大学西京医院儿科脑发育研究室,西安710032 [2]陕西省安康市妇幼保健院新生儿科 [3]第四军医大学西京医院全军中医内科中心

出　　处：《山西医科大学学报》2016年第8期698-702,共5页Journal of Shanxi Medical University

摘　　要：目的探讨琥珀酸(succinic acid,SA)对幼龄大鼠小脑谷氨酸能突触传递的影响。方法采用全细胞膜片钳记录法,在矢状位小脑脑片上记录浦肯野细胞(Purkinje cells,PCs)自发性微小兴奋性突触后电流(miniture excitatory postsynaptic current,m EPSC)和刺激平行纤维(parallel fibre,PF)诱发的PCs兴奋性突触后电位(excitatory postsynaptic potential,EPSP),比较琥珀酸处理前后m EPSC和PF-PC EPSP的变化。结果琥珀酸处理后,幼鼠小脑PCs的自发性mEPSCs幅值显著减小,由给药前的(24.85±2.78)p A减小至给药后的(13.14±0.84)p A,频率也由给药前的(5.04±1.07)Hz降至给药后的(2.77±0.79)Hz,差异均有统计学意义(P<0.01);琥珀酸显著抑制了PF-PC EPSPs的幅值,使其降低至用药前的(37.76±1.10)%(P<0.01),并使EPSP双脉冲(paired-pulse facilitation,PPF)增强的比率较用药前增加了(40.26±2.9)%(P<0.01),差异均有统计学意义。结论琥珀酸对幼龄大鼠小脑谷氨酸能突触传递有显著的抑制作用。Objective To investigate the effects of suceinic acid （SA） on the glutamatergic synaptic transmission in the neonatal rat cerebellum. Methods The whole-cell patch-clamp technique was carried out in Purkinje cells （PCs） of sagittal cerebellar slices to record the spontaneous miniture excitatory postsynaptie current（mEPSC） and the excitatory postsynaptic potential （EPSP） induced by parallel fiber（PF） stimulation. The changes of the mEPSC and the PF-PC EPSP upon SA were analyzed before and after SA perfusion. Results SA significantly reduced the amplitude[ from（24.85 ±2.78）pA to（13.14±0.84）pA,P 〈0.01land the frequency[from （5.04 ± 1.07 ） Hz to （2.77 ± 0.79） Hz, P 〈 0.01 ] of the spontaneous mEPSCs. SA also significantly inhibited PF-PC EPSPs amplitude to （ 37.76 ± 1.10 ） % of the control （ P 〈 0. 01 ） and enhanced the EPSP paired-pulse facilitation （PPF） by （40.26 ± 2.9 ） % （ P 〈 0.01 ）. Conclusion SA may provide an inhibitory effect on cerebellar glutamatergie synaptic transmission in neonatal rats.

关 键 词：琥珀酸 浦肯野细胞 谷氨酸 自发性微小兴奋性突触后电流 兴奋性突触后电位 

分 类 号：R748[医药卫生—神经病学与精神病学]