一种溃疡性结肠炎相关性结肠癌小鼠模型的建立  

Establishment of a mouse model of ulcerative colitis-associated colorectal cancer

作　　者：张瑞[1,2] 朱娜[1] 马静[3] 张文平[4] 刘近春[3] 原丽莉[1] 

机构地区：[1]山西医学科学院,山西医科大学附属山西大医院消化内科,太原030032 [2]山西医科大学第二临床医学院 [3]山西医科大学第一临床医学院 [4]山西医科大学公共卫生学院

出　　处：《山西医科大学学报》2016年第8期720-723,共4页Journal of Shanxi Medical University

基　　金：山西省留学人员管理委员会科研基金资助项目(晋留办发2010-14-97)

摘　　要：目的建立一种简单易行的溃疡性结肠炎相关的结肠癌小鼠模型。方法 5周龄C57BL/6J雄性小鼠被分为实验组(n=10)和对照组(n=7)。实验组小鼠腹腔注射致癌剂氧化偶氮甲烷(AOM)10 mg/kg后给于致炎剂2%葡聚糖硫酸钠(DSS)饮水4 d,之后换为普通饮水17 d为1个周期,共3个周期结束;对照组小鼠腹腔注射等量生理盐水。解剖镜大体观察结肠,并对结肠进行炎症评分、黏膜息肉评分和肿瘤病理评分,评分结果采用非配对t检验,检验水准取ɑ=0.05。结果实验组小鼠腹腔注射AOM后无明显变化,给予DSS后有50%小鼠出现血便,且逐渐加重,试验结束时小鼠表现为反应迟钝,活动减少,出现血便的小鼠均有脱肛。对照组小鼠无异常表现。实验组小鼠较对照组体重增长明显减缓。试验结束时,所有实验组小鼠远端及中段大肠出现不典型增生/癌变,大体解剖镜炎症评分、息肉评分和病理切片肿瘤评分均显著高于对照组(P<0.05)。结论致癌剂AOM和致炎剂DSS可诱导小鼠建立溃疡性结肠炎相关的结肠癌模型。Objective To establish a colitis-associated colorectal cancer mouse model. Methods The 5-week-old C57BL/6J male mice were randomized into experiment group（ n = 10） and control group（ n = 7）. The mice in experiment group were intraperitoneally injected with azoxymethane（ AOM, 10 mg/kg body weight）, and then given the water including 2% DSS for 4 d and finally regular water for 17 d. The process repeated 3 times. The mice in control group were given only regular water. The colon was observed under dissecting microscope, and colon inflammation score, mucosal polyp score and pathological score were evaluated and analyzed with the unpaired t test （a = 0. 05）. Results No obvious changes happened in only AMO-treated mice, but after giving DSS, half of the mice showed the bloody stools and gradually got worse. At the end of experiment, the mice in expriment group showed less activity, and all the mice with bloody stools showed rectal prolapse. The mice in control group had no change. The mice in experiment group showed less weight gain than in control group. At the end of the experiment, all mice showed dysplasia/cancer in middle distal colon in experiment group, and gross anatomy mirror inflammation score, polyp score and pathological tumor rates were significantly higher than in control group （P 〈 0. 05）. Conclusion AOM followed by DSS can effectively induce the formation of colitis-induced colorectal cancer in mice.

关 键 词：溃疡性结肠炎相关性结肠癌 氧化偶氮甲烷 葡聚糖硫酸钠 动物模型 

分 类 号：R735.35[医药卫生—肿瘤]