紫草素对乳腺癌MCF-7细胞雌激素受体的表达和增殖及凋亡的影响  

Effects of shikonin on proliferation,apoptosis and expression of estrogen receptor in breast cancer MCF-7 cells

作　　者：陈玉忠[1] 韩福生[1] 许磊[1] 张智瑞[2] 潘琼[2] 刘浩[2] 金功圣[1] 

机构地区：[1]蚌埠医学院第一附属医院肿瘤外科,蚌埠233000 [2]蚌埠医学院药学系

出　　处：《山西医科大学学报》2016年第8期724-728,共5页Journal of Shanxi Medical University

基　　金：国家自然科学基金资助项目(81372899);安徽省自然科学基金资助项目(1508085MH166)

摘　　要：目的探讨紫草素(shikonin,SK)对乳腺癌MCF-7细胞雌激素受体表达及其对细胞的增殖和凋亡的影响。方法用MTT法检测不同浓度SK(0,1,2,4,8μmol/L)处理MCF-7细胞24,48,72 h的增殖抑制作用;集落克隆形成实验观察低浓度SK(0,0.1,0.2,0.4μmol/L)对MCF-7细胞增殖的影响;DAPI染色观察SK处理24 h对MCF-7细胞凋亡的影响;流式细胞术(PI单染法)检测不同浓度SK(0,1,2,4,8μmol/L)作用MCF-7细胞24 h细胞凋亡比率;Western blot法检测SK对雌激素受体表达的影响。结果 SK作用于MCF-7后细胞存活率随浓度增加而降低(P<0.05)。0.1,0.2,0.4μmol/L SK作用MCF-7细胞后抑制集落克隆形成,0.1,0.2,0.4μmol/L SK组分别与对照组相比,差异均具有统计学意义(P<0.05)。DAPI荧光染色结果显示,随SK浓度(1,2,4,8μmol/L)增加,浓缩深染致密状的细胞核数目逐渐增加。不同浓度的SK(1,2,4,8μmol/L)作用于MCF-7细胞凋亡率分别为(7.0±1.27)%,(11.6±1.45)%,(15.1±1.36)%,(29.5±1.41)%,与对照组相比差异有统计学意义(P<0.05)。MCF-7细胞株中雌激素受体的表达随SK浓度的增加而降低;2,4μmol/L组与对照组相比,差异具有统计学意义(P<0.05)。结论 SK可抑制乳腺癌MCF-7细胞增殖,并诱导其凋亡,其机制可能与雌激素受体表达降低有关。Objective To investigate the effect of shikonin（SK） on the expression of estrogen receptor（ER） and the proliferation and apoptosis in breast cancer cell line MCF-7. Methods MCF-7 cells were exposed to different concentrations（0,1,2,4,8 μmol/L） of SK for 24, 48 and 72 h and the cell viability was assessed with MTI＂ assay. The nuclear changes in MCF-7 cells treated with SK were observed under fluorescent microscope. The apoptotic cell after treated with 0,1,2,4,8 μmol/L SK for 24 h was quantified by flow cytometry. Colony formation assay was used to detect the proliferation of MCF-7 cells after treated with SK. Western blot was used to detect the expression of ER in MCF-7 cells after SK treatment. Results The cell viability of MCF-7 ceils decreased in a concentration- dependent manner after treated with SK（P 〈 0.05 ）. Low levels （0.1,0.2,0.4 μmol/L） of SK inhibited cell colony formation, and the number of colony formation of MCF-7 cells was significantly decreased in 0.1,0.2,0, 4 μmol/L SK groups compared with control group （P 〈0.05）. DAPI staining showed that MCF-7 cell nucleus became condensed and deep-stained gradually after treated with 1,2,4,8 ;xmol/L SK. The apoptotic rate was （7.0± 1.27） %, （ 11.6 ± 1.45 ） %, （ 15.1 ±1.36） % and （29.5 ± 1.41 ） % in MCF-7 cells treated with 1,2,4,8 μmol/L SK, respectively, which was significantly higher than in control group （P 〈 0.05 ）. The expression of ER in MCF-7 cells treated with SK was decreased, and a significant difference was detected in MCF-7 cells exposed to 2, 4 μmol/L SK compared with control group（P 〈 0. 05）. Conclusion SK could inhibit the proliferation and induce the apoptosis in MCF-7 cells, which may be related to down-regulation of the expression of estrogen receptor.

关 键 词：紫草素 乳腺癌 MCF-7细胞 增殖 凋亡 雌激素受体 

分 类 号：R737.9[医药卫生—肿瘤]