醉鱼草有效组分杀灭钉螺的酶促动力学研究（英文）  

Enzyme kinetic analysis of Oncomelania hupensis exposed to active ingredient of Buddleja lindleyana(AIBL)

作　　者：韩邦兴[1] 陈钧[2] 

机构地区：[1]皖西学院生物与制药工程学院,六安237012 [2]江苏大学药学院

出　　处：《中国血吸虫病防治杂志》2016年第4期388-392,共5页Chinese Journal of Schistosomiasis Control

摘　　要：目的分析醉鱼草有效组分(AIBL)对湖北钉螺的酶促动力学影响。方法湖北钉螺浸泡于3.55 mg/L AIBL溶液中,分别于浸泡后24、48、72 h时用赖氏法测定钉螺GPT活性,用化学抑制乳酸底物法测定LDH活性,用磷酸苯二钠比色法测定AKP活性,ELISA法测定ACh E和MDH活性,硫酸甲酯吩嗪反应法测定钉螺SDH活性。结果浓度为3.55 mg/L的AIBL溶液在浸泡湖北钉螺24 h后,钉螺GPT、LDH、AKP活性显著提高,钉螺头足部及肝脏SDH和MDH活性显著降低,但对ACh E活力无影响。结论 AIBL能显著损害湖北钉螺肝脏、作用于无氧和有氧呼吸作用位点、影响能量代谢,并导致能源供应不足,最终导致钉螺死亡。Objective To analyze the enzyme kinetics of active ingredient of Buddleja lindleyana（AIBL）against Oncomelania hupensis,the intermediate host of Schistosoma japonicum. Methods O. hupensis snails were placed in 1 000 ml of 3.55 mg/L AIBL solution for 24,48 h and 72 h,respectively,and the enzyme kinetics of alanine aminotransferase（GPT）was determined by ReitmanFrankel assay,lactate dehydrogenase（LDH）by the chemical inhibition lactic acid substrate method,alkaline phosphatase（AKP）by the disodium phenyl phosphate colorimetric method,acetylcholine esterase（AChE）and malate dehydrogenas（MDH）by ELISA,and succinate dehydrogenase（SDH）by the phenazine methyl sulfate reaction method（PMS）in the soft tissues of O. hupensis before and after AIBL treatment. Results Following exposure to 3.55 mg/L AIBL solution for 24 h, the GPT,LDH,and AKP activities significantly improved in the soft tissues of O. hupensis,while the SDH and MDH activities were significantly lowered in the headfoot and liver. However,AIBL treatment did not cause significant effect on AChE activity in O. hupensis. Conclusions AIBL causes significant damages to O. hupensis liver and can efficiently act on anaerobic and aerobic respiration loci,which will hinder energy metabolism,and cause inadequate energy supply in cells used for normal secretion,eventually leading to O. hupensis death.

关 键 词：湖北钉螺 醉鱼草 酶促动力学 

分 类 号：R383.24[医药卫生—医学寄生虫学]