粪便微RNA-141、-17-3p和-92a-3p对结直肠癌诊断及预后判断的价值  

Values of fecal microRNA-141,-17-3p and-92a-3p in the diagnosis and prognostic evaluation of colorectal cancer

作　　者：薛亚晶[1] 陈建[2] 张金业[1] 朱自力[1] 吴晓燕[1] 林兰[1] 

机构地区：[1]南通市肿瘤医院检验科,江苏南通226600 [2]南通市第三人民医院消化内科,江苏南通226600

出　　处：《肿瘤》2016年第8期901-907,共7页Tumor

摘　　要：目的：探讨粪便微RNA（mirco RNA,mi RNA,mi R）对结直肠癌诊断及预后判断的价值。方法 ：纳入50例结直肠癌患者和50例健康对照者。实时荧光定量PCR法检测所有受试者术前粪便中mi R-141、mi R-17-3p和mi R-92a-3p的含量,分析这3种mi RNA在2组之间的差异,并应用受试者工作特征（receiver operating characteristic,ROC）曲线评估3种mi RNA作为结直肠癌诊断指标的效能。分析结直肠癌患者术前及术后6个月时粪便中mi R-141和mi R-92a-3p含量的差异;跟踪随访结直肠癌患者在术后12个月内的复发情况,再分析复发患者与未复发患者粪便中mi R-141含量在术前与术后6个月时差值的差异。结果 ：结直肠癌组术前粪便中mi R-141和mi R-92a-3p含量均明显高于健康对照组（P值均〈0.01）,而mi R-17-3p含量在两组之间无明显差异（P=0.30）。ROC曲线分析发现,粪便mi R-141的曲线下面积（area under the curve,AUC）为0.874[95%可信区间（confidence interval,CI）为0.803~0.946）,粪便mi R-92a-3p的AUC为0.789（95%CI为0.703~0.876）。患者术后6个月时粪便miR-141含量明显低于术前（P〈0.01）,而术后mi R-92a-3p含量与术前相比无明显差异（P=0.08）。对于4例术后12个月内确诊复发的患者,术前与术后6个月时粪便mi R-141含量的差值明显小于46例未复发患者（P〈0.05）。结论 ：结直肠癌患者粪便中mi R-141和mi R-92a-3p的含量均明显升高,提示它们可以作为诊断结直肠癌的生物学指标;而且粪便mi R-141对评价结直肠癌的手术效果和预后以及监测术后复发有一定价值。Objective： To explore the values of fecal mircoRNA （miRNA, miR） in diagnosis and prognostic evaluation of colorectal cancer Methods： Fifty colorectal cancer patients and fifty healthy controls were included in this study. The contents of fecal miR-141, miR-17-3p and miR-92a-3p in all subjects were detected by real-time fluorescent quantitative PCR before operation. The differences of the three mircoRNA levels between the two groups were analyzed. Then the diagnostic efficiency of the three miRNAs for colorectal cancer was assessed by using receiver operating characteristic （ROC） curve. Furthermore, the differences of fecal miR-141 and miR-92a-3p levels in colorectal cancer patients between preoperation and postoperation （6 months after operation） were analyzed. The recurrence of colorectal cancer patients in 12 months after operation was followed up, then the differences between postoperative and preoperative fecal miR-141 contents in patients with and without recurrence were analyzed. Results： The levels of fecal miR-141 and miR-92a-3p in colorectal cancer group were significantly higher than those in the control group （both P 〈 0.01）. There was no significant difference in fecal miR-17-3p level between the two groups （P = 0.30）. ROC curve analysis showed that the areas under the curve （AUC） of fecal miR-141 and miR-92a-3p were 0.874 [95% confidence interval （C/）： 0.803-0.946] and 0.789 （95% CI： 0.703-0.876）, respectively. The level of fecal miR-141 in colorectal cancer patients 6 months after operation was significantly lower than that before operation （P 〈 0.01）, while there was no significantly difference between postoperative and preoperative fecal miR-92a-3p levels （P = 0.08）. The difference between postoperative and preoperative fecal miR-141 contents in 4 patients with recurrence was significantly less than that in 46 patients without recurrence （P 〈 0.05）. Conclusion： The fecal miR-141 and miR-92a-3p were highly expressed in patients with colo

关 键 词：结直肠肿瘤 粪便 微RNAS 早期诊断 预后 

分 类 号：R735.34[医药卫生—肿瘤]