基于EST-SSR标记的云南黄瓜种质资源遗传多样性分析  

Analysis on Genetic Diversity of Local Cucumber Germplasm Resources in Yunnan Based on EST-SSR Markers

作　　者：李翔[1] 张颖[1] 许俊强[1] 宋婷[1] 李文璐[1] 董玉梅[1] 张应华[1] 

机构地区：[1]云南农业大学云南省滇台特色农业产业化工程研究中心,昆明650201

出　　处：《分子植物育种》2016年第5期1179-1188,共10页Molecular Plant Breeding

基　　金：云南省级财政发展生物产业专项资金项目((2013)-313);云南农业大学研究生科技创新项目(2015ykc30)共同资助

摘　　要：本研究从87对黄瓜EST-SSR引物中筛选出64对多态性引物,对39份云南地方黄瓜材料进行了遗传多样性和亲缘关系分析,281个观察等位基因被检测,平均检测每对引物4.40个,平均Shannon's信息指数为1.08。PIC在0.07~0.84之间,平均为0.55,Ho在0.05~0.92之间,均值为0.56,表明云南地方黄瓜种质资源遗传多样性较丰富。采用非加权类平均法(UPGMA)聚类,聚类分析表明39份云南地方黄瓜材料,当SM为0.665(L1)时,分为3大类群;当SM为0.738(L2)时,分为5个亚类群。二维图主坐标分析方法将其分为4大类群,而三维图主坐标分析方法分为6个亚类群。聚类分析和主坐标分析所获得结果基本一致,三维图主坐标更能较为真实的反映39个云南地方黄瓜材料间的亲缘关系,研究结果可为黄瓜种质资源保存和新品种选育提供依据。In this study, 64 polymorphic primer pairs of EST-SSR markers, which were selected from 87, were used to amplify the genomic DNA isolated from 39 cucumbers in Yunnan genotypes in order to analyze their genetic diversity and relationship. The results showed that 281 alleles were amplified. The average number of alleles detected by a pair of primer was 4.40. The average of Shannon＇s information index was 1.08. The polymor- phism information content （PIC） varied from 0.07 to 0.84 and the observed heterozygosity （Ho） ranged from 0.05 to 0.92, with an average of 0.55 and 0.56, respectively. In summary, the genetic diversity of cucumber germplasm resources in Yunnan was rich. Then Cluster analysis results by unweighted pair-group method arithmetic average （UPGMA） showed that the Dice similarity coe^cients of 39 cucumber germplasms ranged from 0.61 to 0.93, whi- ch indicated that the phylogenetic relationships were relatively far. The 39 cucumber accessions could be divided into 3 groups and 5 subgroups at the level ofSM--0.665 （L1） and 0.738 （L2） by cluster analysis, respectively. The accessions were sorted into 4 groups and 6 subgroups by two-dimensional and three-dimensional principal coordinate analysis, respectively. The cluster analysis and the principal coordinate analysis exhibited similar phylogenesis among the tested materials. However, more authentic genetic relationship among 39 cucumber accessions could be provided by three-dimensional principal coordinates analysis. The analysis results would not only be of great help to conserve the cucumber germplasms but also provide theoretical information for molecular breeding.

关 键 词：黄瓜 聚类分析 主坐标分析 种质资源 

分 类 号：S642.2[农业科学—蔬菜学]