口腔鳞癌细胞分泌的胞外体对其生物学行为的影响  

Effects of Exosomes Secreted by Oral Squamous Cell Carcinoma Cells on Their Biological Behaviors

作　　者：李宠[1] 朱杨[1] 山本哲也 李德超[1] 

机构地区：[1]佳木斯大学附属口腔医院颌面外科,黑龙江佳木斯154007 [2]高知医科大学口腔颌面外科,高知783-8505

出　　处：《口腔医学研究》2016年第8期800-803,共4页Journal of Oral Science Research

基　　金：黑龙江省卫生厅课题(项目编号:2013-218)

摘　　要：目的:研究口腔鳞状细胞癌细胞细胞株(OSC-4)分泌的胞外体对细胞生物学行为的影响。方法:培养OSC-4,从其上清培养液中分离提纯胞外体(exosome,EXO),CD9标记,将提取的胞外体作用于OSC-4,MTT检测胞外体对OSC-4增殖能力的影响,细胞划痕实验检测胞外体对OSC-4迁移能力的影响,transwill小室侵袭实验检测胞外体对OSC-4侵袭能力的影响。结果:细胞上清液提取物免疫金标结果可见金标记的CD9,即分离提纯物为胞外体;MTT检测结果显示胞外体作用后的OSC-4的增殖能力增强(P<0.05),细胞划痕实验结果显示其迁移能力增强和transwill小室侵袭实验结果显示其侵袭能力也增强(P<0.05)。结论:OSC-4分泌的胞外体可以促进口腔鳞状细胞癌细胞的增殖、迁移、浸润能力。Objective：To study the effectsof exosomes secreted byoral squamous cell carcinoma cells（OSC-4）on their biological behaviors.Methods：Exosomesderived from OSC-4cells were separated from the medium.The effects of exosomes on the OSC-4cell proliferation,migration and invasion were detected by MTT,wound healing,andtranswellassays,respectively.Results：Gold-labeling CD9 was detected in the isolated exosomes.MTT assay showed that the OSC-4cells proliferation ability was increased after treated with exosomes（P0.05）.Wound healing and transwell assays results showed that the migration and invasion ability of OSC-4cells were also increased after treated byexosomes（P0.05）.Conclusion：Exosomes secreted byoral squamous cell carcinomacells couldpromote their proliferation,migration,and invasion.

关 键 词：口腔鳞状细胞癌 胞外体 生物学行为 

分 类 号：R739.8[医药卫生—肿瘤]