钴离子对人肾小管上皮细胞毒性及Bax、Bcl-2基因表达变化的实验研究  

Effect of Co^(2+) on the Cytotoxicity of Human Renal Tubular Epithelial Cells and Gene Expression of Bax and Bcl-2

作　　者：罗新年 连文伟[1] 

机构地区：[1]南昌大学附属口腔医院修复科,江西南昌330006

出　　处：《口腔医学研究》2016年第8期869-873,共5页Journal of Oral Science Research

摘　　要：目的:研究Co^(2+)对人肾小管上皮细胞的毒性及对凋亡基因Bax、Bcl-2表达变化的影响。方法:体外培养肾小管上皮细胞,以5种浓度(0~10μmol/L)的Co^(2+)培养液培养细胞12h、24h、48h。倒置显微镜下观察和记录细胞生长状态,采用MTT法检测各培养组在不同时间点的细胞增殖率,RT-PCR法检测各培养组在不同时间点Bax、Bcl-2的表达变化,以评价Co^(2+)的细胞毒性。结果:当Co^(2+)作用浓度在7.5μmol/L以下,观察时间内对细胞无毒性;在7.5μmol/L以上时,刺激24h以后对细胞有轻度毒性。随着Co^(2+)作用浓度和时间的增加,各实验各组Bax mRNA表达量逐渐上调,Bcl-2mRNA表达量逐渐下调,Bax/Bcl-2的比值升高。结论:Co^(2+)抑制肾小管上皮细胞的增殖,低浓度Co^(2+)对肾小管上皮细胞不存在毒性作用,高浓度时(≥7.5μmol/L对细胞有轻度毒性,诱导细胞发生凋亡。Objective：To study the effect of Co^（2＋）on the cytotoxicity of human renal tubular epithelial cells and the gene expression of Bax and Bcl-2.Methods：HK-2cells were cultured and incubated with five different concentrations of Co^（2＋）solution（0-10μmol/L）for 12 h,24hand 48 hin vitro.Cells were observed and recorded under inverted phase contrast microscope.Cell proliferation of each culture group was detected by MTT and the expression of Bax and Bcl-2were detected by RT-PCR at different time points.Results：Co^（2＋）had no toxicity on HK-2cells when the concentration was lower than 7.5μmol/L during the observation time.While when the concentration was higher than 7.5μmol/L,Co^（2＋）had mild toxicity on HK-2cells after 24 h.As to the increase of Co^（2＋）concentration and time of duration,the mRNA expression of Bax in each experimental group gradually increased,while the mRNA expression of Bcl-2gradually decreased and the value of Bax/Bcl-2increased.Conclusion：Co^（2＋）inhibited the proliferation of HK-2cells.It has no toxicity on human renal tubular epithelial cells at low concentration,but has mild toxicity and promotes cell apoptosis at high concentration（≥7.5μmol/L）.

关 键 词：钴离子 人肾小管上皮细胞 细胞毒性 

分 类 号：R783.1[医药卫生—口腔医学]