胆管癌患者调节性T细胞及白介素-35的表达及临床意义  

The expressions and the clinical significance of Treg and IL-35 in patients with cholangiocarcinoma

作　　者：王义真 刘浩润[2] 肖漓[2] 赵洪强[2] 刘航[2] 崔洪涛[2] 李为民[2] 

机构地区：[1]河北北方学院,075000 [2]解放军第309医院肝胆外科

出　　处：《中华肝胆外科杂志》2016年第8期542-545,共4页Chinese Journal of Hepatobiliary Surgery

摘　　要：目的探讨胆管癌患者外周血及肿瘤组织中调节性T细胞（Treg）及相关细胞因子白介素-35（IL-35）的表达及临床意义。方法分别采用流式细胞技术、PCR、酶联免疫吸附法及免疫组化对42例胆管癌患者外周血及肿瘤组织中Treg细胞及IL-35表达进行检测。与正常人比较，分析Treg细胞及IL-35在胆管癌患者中的变化水平及临床意义。结果胆管癌患者外周血中Treg细胞占CD4^T细胞的比例为（5．6±1．7）％，对照组为（2．9±0．8）％，两组差异有统计学意义（P〈0．05）。胆管癌患者外周血浆IL-35水平为（198．4±81．4）pg／ml，对照组IL-35为（33．7±18．0）pg／ml，两组差异有统计学意义（P〈0．05）。外周血单核细胞（PBMC）中，IL-35两个亚基EBI3和p35的mRNA相对表达水平与血浆IL-35表达水平呈正相关（p35，R=0．795，P〈0．05；EBI3，R=0．812，P〈0．05）。免疫组化结果提示胆管癌组织中FOXP3^＋癌细胞及Treg细胞明显增多。结论Treg和IL-35在胆管癌患者外周血及肿瘤组织中表达增高，可能与胆管癌的发生发展有关。Objective To investigate the expressions of regulatory T cell （Treg） and interleukin-35 （IL-35） in patients with eholangiocarcinoma and to explore their clinical significance. Methods Flow-eytometry, PCR, Enzyme-linked immunosorbent assay （Elisa） and immunohistoehemistry were used to detect the levels of Treg and IL-35 in peripheral blood and eholangiocarcinoma tissues in 42 patients with cholangiocarcinoma. Healthy volunteers were used as a control group. Result The percentage of Treg cells to CD4 ^＋ T cells in patients with cholangiocarcinoma was （5.6 ± 1.7 ） % , while that in the normal control group was （ 2.9 ±0.8 ） %. There was a significant difference between the two groups （ P 〈 0.05 ）. The plasma levels of IL-35 in patients with eholangioearcinoma was （ 198.4 ± 81.4） pg/ml, while that in the normal control group was （33.7 ± 18.0） pg/ml. Again, a significant difference was observed between the two groups （P 〈 0. 05 ）. In peripheral blood mononuclear cell, the IL-35 mRNA level was positively correlated with the plasma IL-35 level （p35, R = 0.795, P 〈 0.05 ; EBI3, R = 0. 812, P 〈 0.05）. Immunohistoehemieal studies showed that FOXP3 ＋ tumor cells and Treg cells increased significantly in tumor tissues. Conclusion Overexpressions of Treg and IL-35 in peripheral blood and tumor tissues of patients with cholangioeareinoma suggested that they may play important roles in the development of cholangiocarcinoma.

关 键 词：胆管癌 调节性T细胞 白介素-35 

分 类 号：R735.8[医药卫生—肿瘤]