芹菜素通过抑制Src通路抑制黑色素瘤细胞增殖  

Apigenin Inhibits the Proliferation of A375 Cells by Suppressing the Src Pathway

作　　者：郭冰玉[1] 张宇[1] 回蔷[1] 常鹏[1] 陶凯[1] 

机构地区：[1]沈阳军区总医院整形外科,沈阳110840

出　　处：《中国医科大学学报》2016年第9期813-817,共5页Journal of China Medical University

摘　　要：目的探讨芹菜素如何通过调节Src通路来抑制黑色素瘤细胞增殖。方法采用不同浓度的芹菜素处理黑色素瘤细胞系A375,使用HEK293细胞系(正常细胞系)作为对照,通过MTT实验观察芹菜素是否可以特异性抑制肿瘤细胞的增殖。进一步通过Western blot以及实时PCR检测芹菜素对A375细胞中Src通路增殖相关蛋白CDK4和CDK6的影响。结果 MTT检测结果发现芹菜素对HEK293细胞的增殖抑制作用不明显,但是10μmol/L、20μmol/L、40μmol/L与80μmol/L的芹菜素分别作用于A375细胞后对细胞增殖的抑制率分别为(22.12±7.20)%、(27.08±4.37)%、(37.39±2.72)%和(39.00±1.57)%,而且随着药物的浓度增加对A375细胞增殖的抑制率增加。Western blot和实时PCR检测发现,20μmol/L、40μmol/L与80μmol/L的芹菜素均可以抑制A375细胞中Src通路下游CDK4和CDK6蛋白的表达。结论芹菜素可以通过抑制Src通路显著抑制黑色素瘤细胞系A375的增殖,对正常细胞作用较小,可以作为潜在的化疗药物应用于黑色素瘤的化疗。Objective To investigate whether Apigenin can inhibit the proliferation of A375 cells by suppressing the Sre pathway. Methods HEK 293 cells （as control） and A375 cells were treated with different concentrations of Apigenin. MTT assay was used to observe whether Apigen- in could inhibit the proliferation of A375 cells. Western blot and real-time PCR were done to test the effect of Apigenin on CDK4 and CDK6 expres- sion. Results After treated with 10 μmol/L, 20 μmol/L, 40μmol/L and 80 μmol/L of Apigenin, the inhibition rate of proliferation on A375 cells were 22.12%±7.20%, 27.08%±4.37%, 37.39%±2.72%, and 39.00%± 1.57%, respectively. It was found that the inhibition rate was increased with the concentration of the drug; however, there was no significant inhibition on HEK293 cells. Western blot and real-time PCR showed that compared with the control group, 20 μmol/L, 40 μmol/L and 80 μmol/L of Apigenin could significantly inhibit the expressions of CDK4 and CDK6 in A375 cells. Conclusion Apigenin can significantly inhibit the proliferation of A375 cells by suppressing the Src pathway, but its role in HEK293 cells is smaller.

关 键 词：芹菜素 黑色素瘤 A375 增殖 SRC 

分 类 号：R739.5[医药卫生—肿瘤]