表没食子儿茶素没食子酸酯对育肥猪骨骼肌纤维类型的影响  

Effects of EGCG on Muscle Fiber Types of Finishing Pigs

作　　者：王丽娜[1] 王珍[1] 彭建龙[1] 王建兵[1] 杨柯林[1] 束刚[1] 王松波[1] 朱晓彤[1] 高萍[1] 江青艳[1] 

机构地区：[1]华南农业大学动物科学学院,国家生猪种业工程技术研究中心,广州510642

出　　处：《畜牧兽医学报》2016年第8期1581-1591,共11页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家重点基础研究发展计划(2012CB124701);国家自然科学基金青年基金(31101780);博士点基金新教师类(20114404120001)

摘　　要：本试验主要研究在饲粮中添加表没食子儿茶素没食子酸酯(Epigallocatechin gallate,EGCG)对育肥猪肌纤维类型的影响。试验选用156日龄"杜×长×大"三元杂交育肥猪180头,平均体重为(85.02±1.15)kg,按组间体重、性别比例一致的原则,将育肥猪随机分成3组,每组6个重复,每个重复10头猪(5公5母)。对照组饲喂基础饲粮,试验组饲喂在基础饲粮上分别添加0.025%、0.05%EGCG的试验饲粮。研究结果表明:1)育肥猪饲粮中添加0.025%的EGCG,能显著下调背最长肌乳酸脱氢酶(Lactate dehydrogenase,LDH)的活性(P<0.05),减少MyHCⅠ、PGC-1α和mtTFA蛋白水平和MyHCⅠ、Tnnt 1、Cytc和COXⅣmRNA的表达(P<0.05);显著下调腰大肌LDH和琥珀酸脱氢酶(Succinate dehydrogenase,SDH)的活性(P<0.05),显著上调Ⅰ型肌纤维比例(P<0.05),显著下调MyHCⅠmRNA的表达(P<0.05),而MyHCⅡa、MyHCⅡx、MyHCⅡb的mRNA显著上调(P<0.05)。2)育肥猪饲粮中添加0.050%的EGCG,能显著下调背最长肌LDH和SDH的活性(P<0.05),降低活性氧(Reactive oxygen species,ROS)水平(P<0.05),抑制AMPK的磷酸化、下调MyHCⅠ、PGC-1α、mtTFA和NRF-1的蛋白和mRNA表达(P<0.05);显著下调腰大肌LDH和SDH的活性(P<0.05),降低ROS水平(P<0.05),下调MyHCⅠ蛋白和mRNA的表达、抑制AMPK的磷酸化和NRF-1的蛋白表达(P<0.05),显著上调Ⅰ型肌纤维比例(P<0.05)。综上,育肥猪饲粮中添加EGCG后,可能通过EGCG清除ROS后引起AMPK活性减弱,降低PGC-1α表达,最终导致线粒体生物合成下降和慢肌纤维形成减少。The aim of this study was to investigate the effects of Epigallocatechin Gallate（EGCG）on muscle fiber types of finishing pigs.One-hundred eighty crossbred finishing pigs（Duroc ×Yorkshire× Landrace,156-day-old）with an average body weight of（85.02±1.15）kg were selected for this study.According to the consistent principle of weight and sex ratio between groups,pigs were randomly divided into 3groups,and each group with 6repeats（10pigs/repeat（5male,5female））.Control group was fed with basal diet,treatment groups were fed with basal diet supplemented with 0.025% and 0.05% EGCG,respectively.The results showed that：1）0.025% EGCG could significantly decrease the activity of LDH,the protein level of MyHCⅠ,PGC-1α,mtTFA and mRNA expression of MyHCⅠ,Tnnt 1,Cytc and COXⅣin pigs longissi-mus dorsi（P〈0.05）;in psoas major muscle,the activity of LDH and SDH and mRNA expression of MyHC Ⅰwas reduced,the proportion of typeⅠmuscle fiber and mRNA expression of MyHCⅡa,MyHCⅡx,MyHCⅡb were upregulated（P〈0.05）.2）In longissimus dorsi,0.05% EGCG treatment could significantly decrease the activity of LDH and SDH（P〈0.05）,the level of ROS（P〈0.05）,and activity of AMPK,the level of protein and mRNA expression of MyHCⅠ,PGC-1α,mtTFA and NRF-1（P〈0.05）.While,in psoas major muscle,0.050% EGCG treatment reduced the activity of LDH and SDH（P〈0.05）,the level of ROS（P〈0.05）,and the protein level and mRNA expression of MyHCⅠ,inhibited the phosphorylation of AMPK and protein expression of NRF-1（P〈0.05）,but the proportion of typeⅠmuscle fiber was upregulated.In conclusion,the effect of EGCG on longissimus dorsi and psoas major of pigs might be completed by reducing ROS level,thus decreasing the AMPK activity and the expression of PGC-1α,eventually reducing the formation of slow-twitch muscle fibers and mitochondria biosynthesis.

关 键 词：EGCG 育肥猪 肌纤维类型 线粒体生物合成 

分 类 号：S828[农业科学—畜牧学] S821.2[农业科学—畜牧兽医]