CML患者TGF-β1 mRNA与bcr/abl^(P210)融合基因转录本表达的关系  

Relationship between TGF-β1 mRNA and bcr/abl^(P210) fusion gene transcription expression levels in CML patients

作　　者：许大兵 孙余婕[1] 沈佐君[1] 

机构地区：[1]安徽医科大学附属省立医院安徽省临床检验中心,安徽合肥230001

出　　处：《检验医学》2016年第8期671-674,共4页Laboratory Medicine

基　　金：国家自然科学基金项目(30672011);安徽省卫生厅医学课题(13cz026)

摘　　要：目的通过分析慢性粒细胞白血病(CML)患者转化生长因子-β1(TGF-β1)mRNA与bcr/abl^(P210)融合基因转录本(简称bcr/abl^(P210))的表达水平,探讨两者在CML发病过程中可能的作用。方法采用实时荧光定量聚合酶链反应(PCR)检测73例CML患者外周血和骨髓中TGF-β1 mRNA及bcr/abl^(P210)的表达水平,以22名健康体检者作为正常对照组。TGF-β1 mRNA与bcr/abl^(P210)的相关性采用Pearson相关分析。结果 CML患者骨髓和外周血样本中TGF-β1 mRNA、bcr/abl^(P210)的表达水平差异无统计学意义(P>0.05)。正常对照组外周血中TGF-β1 mRNA的表达水平明显高于CML患者骨髓中TGF-β1 mRNA的表达水平(P<0.05),正常对照组TGF-β1 mRNA的表达水平是CML组的11.39倍。相关性分析显示CML患者TGF-β1 mRNA与bcr/abl^(P210)呈正相关(r=0.53,P<0.01)。结论骨髓TGF-β1 mRNA有可能作为一种新的CML诊疗指标。Objective To investigate the possible pathogenesis of chronic myelogenous leukemia（CML） by analyzing the expression levels of transforming growth factor-beta 1（TGF-β1） mRNA and bcr/ablP210 fusion gene transcription in patients with CML. Methods Real-time fluorescence quantitative polymerase chain reaction （PCR） was used to determine the expression levels of TGF-β1 mRNA and bcr/ablP210 in peripheral blood and bone marrow of 73 patients with CML. A total of 22 healthy subjects were enrolled as healthy control group. Pearson correlation analysis was used to analyze the correlation of TGF-β1 mRNA and bcr/ablP210. Results The expression levels of TGF-β1 mRNA and bcr/ablP210 in peripheral blood and bone marrow of patients with CML had no statistical significance（P〉0.05）. The expression levels of TGF-β1 mRNA in healthy control group were higher for 11.39 times than those in CML group（P〈0.05）. The expression levels of TGF-β1 mRNA and bcr/ablP210 had a positive correlation in CML group（r=0.53,P〈0.01）. Conclusions TGF-β1 mRNA in bone marrow may be a new indicator for the diagnosis and treatment of CML.

关 键 词：转化生长因子-β1 bcr/ablP210 融合基因 转录本 

分 类 号：R446.1[医药卫生—诊断学]