青蒿素和顺铂联合干预对胃癌MGC-803细胞株上皮间质转化、血管新生及ATP生成的影响  

Effect of artemisinin combined with cisplatin intervention on epithelial-mesenchymal transition,angiogenesis and ATP generation in MGC-803 gastric cancer cell lines

作　　者：吴孟轩[1] 

机构地区：[1]四川省自贡市第三人民医院药剂科,四川自贡643020

出　　处：《海南医学院学报》2016年第18期2073-2076,共4页Journal of Hainan Medical University

基　　金：四川省卫生厅科研课题(130021)~~

摘　　要：目的:研究青蒿素和顺铂联合干预对胃癌MGC-803细胞株上皮间质转化、血管新生及ATP生成的影响。方法:培养胃癌MGC-803细胞株并分为对照组、顺铂组、青蒿素组、顺铂+青蒿素组、顺铂+青蒿素+NAC组、NAC组,给予不同的条件处理后测定细胞活力、凋亡率以及上皮间质转化分子、血管新生分子、ATP的含量。结果:顺铂组、青蒿素组、顺铂+青蒿素组的细胞活力值、ATP生成量以及VEGFA、VEGFB、VEGFC、N-cadherin、Vimentin含量低于对照组,早期凋亡率、晚期凋亡率以及E-cadherin含量高于对照组;顺铂+青蒿素组的细胞活力值、ATP生成量以及VEGFA、VEGFB、VEGFC、N-cadherin、Vimentin含量低于顺铂组和青蒿素组,早期凋亡率、晚期凋亡率以及E-cadherin含量高于顺铂组和青蒿素组。顺铂+青蒿素+NAC组的E-cadherin含量低于顺铂+青蒿素组,ATP生成量以及VEGFA、VEGFB、VEGFC、N-cadherin、Vimentin含量高于顺铂+青蒿素组。结论:青蒿素和顺铂联合干预能够协同发挥对胃癌MGC-803细胞株上皮间质转化、血管新生及ATP生成的抑制效应,该抑制效应部分通过增加活性氧的生成来发挥。Objective： To study the effect of artemisinin combined with cisplatin intervention on epithelial-mesenchymal transition,angiogenesis and ATP generation in MGC-803 gastric cancer cell lines. Methods： MGC-803 gastric cancer cell lines were cultured and divided into control group,cisplatin group,artemisinin group,cisplatin ＋ artemisinin group,cisplatin ＋ artemisinin ＋NAC group and NAC group,and after different conditions of treatment,cell viability,apoptosis rate as well as levels of epithelial-mesenchymal transition molecules,angiogenesis molecules and ATP were determined. Results： Cell viability,ATP generation as well as VEGFA,VEGFB,VEGFC,N-cadherin and Vimentin levels of cisplatin group,artemisinin group and cisplatin ＋ artemisinin group were lower than those of control group,and early apoptosis rate,late apoptosis rate and E-cadherin levels were higher than those of control group; cell viability,ATP generation as well as VEGFA,VEGFB,VEGFC,N-cadherin and Vimentin levels of cisplatin ＋ artemisinin group were lower than those of cisplatin group and artemisinin group,and early apoptosis rate,late apoptosis rate and E-cadherin level were higher than those of cisplatin group and artemisinin group. E-cadherin level of cisplatin ＋ artemisinin ＋ NAC group was lower than that of cisplatin ＋ artemisinin group,and ATP generation as well as VEGFA,VEGFB,VEGFC,N-cadherin and Vimentin levels were higher than those of cisplatin ＋ artemisinin group. Conclusion： Artemisinin combined with cisplatin intervention cansynergistically exert the inhibitory effect on epithelial-mesenchymal transition,angiogenesis and ATP generation in MGC-803 gastric cancer cell lines,and the inhibiting effect is partially realized by increasing the generation of reactive oxygen species.

关 键 词：胃癌 青蒿素 顺铂 上皮间质转化 血管新生 三磷酸腺苷 活性氧簇 

分 类 号：R735.2[医药卫生—肿瘤]