益生菌及人体肠道菌群对低聚甘露糖的降解  

Utilization of mannan-oligosaccharides by probiotics and human intestinal microflora

作　　者：陈秀霞[1,2] 尹业师[2] 朱立颖[2] 皮雄娥[2] 刘伟[2] 王欣[2] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321000 [2]浙江省农业科学院,浙江杭州310000

出　　处：《中国微生态学杂志》2016年第7期745-750,共6页Chinese Journal of Microecology

基　　金：国家高技术研究发展计划(863计划)(2015AA0-21501)

摘　　要：目的研究益生菌及肠道菌群对低聚甘露糖的降解情况。方法(1)将5名志愿者粪便样品,13株纯菌以及7种微生态制剂接种到VI-MO液体培养基中,37℃厌氧培养24或72h,取样并采用TLC(薄层层析)检测降解情况;(2)选取7种微生态制剂中降解能力较强的乐塞益生菌胶囊(LS)中的益生菌作为筛选源,采用VI-MO固体培养基分离纯化低聚甘露糖的单一降解菌;(3)将B.uniformis L8和L.plantarum LS1A同时接种到VI-MO液体培养基中,37℃厌氧培养72h,取样并采用TLC检测降解情况。结果 (1)不同人体肠道菌群和微生态制剂对低聚甘露糖的利用情况存在较大差异,B.uniformis L8和B.xylanisolvens C5可利用大聚合度低聚甘露糖;(2)从微生态制剂中分离得到的L.plantarum LS1A可利用小聚合度低聚甘露糖;(3)B.uniformis L8利用MO产生的小寡糖能被L.plantarum LS1A利用。结论低聚甘露糖被拟杆菌降解释放出的小聚合度寡糖可以被乳酸菌利用,作为益生元很有可能改善肠道菌群的结构。Objective To investigate the degradation of Mannose oligosaccharides （MOs） by probiotics and human intestinal microflora. Methods Five fecal samples collected from 5 volunteers and seven commercial probiotic products were inoculated into liquid VI medium containing MOs （VI-MOs） respectively. Meanwhile, thirteen bacterial strains were inoculated into VI-MO medium. After cultivated anaerobically, the degradation of MOs was tested with thin layer chromatography （TLC）. The MO-degrading strains, B. uniformis L8 and L. plantarum LS1A, were then isolated and identified, and the two MO-degrading strains were co-cultured in VI-MOs medium to test their ability of MO degradation. Results The ability of MO degradation by different human intestinal flora varied, and only B. uniformis L8 and B. xylanisolvens C5 showed the ability to degrade MOs in vitro. The L. plantarum LS1A isolated from probiotic products could utilize MOs with low polymerization. When co-cultured in VI-MOs medium, the small fragments produced by B. uniformis L8 was further utilized by L. plantarum LS1A. Conclusion Human intestinal bacteria can utilize MOs, and Bacteroides is the primary MO degrader. The small fragments produced by Bacteroides can be further utilized by Lactobacilli. Therefore, MOs can be classified as one of prebiotics to improve human gut health.

关 键 词：益生菌 肠道菌群 低聚甘露糖 拟杆菌 乳酸菌 

分 类 号：Q939.9[生物学—微生物学]