MRSA大鼠感染性心内膜炎模型的建立  

Establishment of MRSA infected endocarditis model in rats

作　　者：白羽[1] 李雪[1] 杨信怡[1] 李聪然[1] 卢曦[1] 胡辛欣[1] 聂彤影 庞晶[1] 王秀坤[1] 游雪甫[1] 

机构地区：[1]中国医学科学院北京协和医学院医药生物技术研究所药理室,北京100050

出　　处：《中国医药生物技术》2016年第4期289-294,共6页Chinese Medicinal Biotechnology

基　　金："十二五"国家科技重大专项(2012ZX09301002-001;2012ZX09301002-005;2014ZX09507009-012);北京市重点实验室科技创新基地培育与发展工程(Z141102004414065);高等学校博士学科点专项科研基金(20121106120027)

摘　　要：目的选用ATCC43300标准菌株,建立MRSA感染的大鼠心内膜炎模型。方法大鼠经右侧颈总动脉插管至左心室,术后24 h,尾静脉注射10^3、10^4和10^5 CFU/只感染量的ATCC43300菌液,分别在感染后的1、3、5和7 d处死大鼠,采集心脏赘生物、脾脏和肾脏进行菌落计数,同时设非手术感染组和手术非感染组作为对照。结果感染1 d后,心脏插管大鼠瓣膜处均有赘生物形成,严重者甚至形成壁性赘生物,且随感染菌浓度的增加,心内膜炎感染程度加剧。10^3 CFU/只感染量组、10^4 CFU/只感染量组、10^5 CFU/只感染量组,赘生物细菌计数的对数值分别为7.04±1.38、9.32±1.22、9.78±1.03;感染3 d后,细菌性心内膜炎感染最为严重,细菌计数的对数值最高可达10.03±0.71;随后的5^7 d,感染程度随感染量均有不同程度的下降,10^3 CFU/只感染量组和10^4 CFU/只感染量组,分别下降至6.87±0.63和8.23±1.05;脾脏和肾脏的感染趋势与心脏大致相同,但感染程度低于心脏。手术非感染组和非手术感染组则无赘生物的形成。结论应用该方法建立的模型具有可操作性强、简单、稳定、重复性好和成功率高的特点,更加适用于抗菌药物机制、新药筛选评价及心内膜炎相关性疾病的研究。Objective To establish an rat model of Staphylococcus aureus（MRSA） infected endocarditis（IE）by using the ATCC43300 standard strains. Methods Valve trauma was produced by introduction of an indwelling PE10 catheter into the aortic valve via the left carotid artery. After operation, the ATCC43300 standard strains were injected into the lateral tail vein within 24 hours, and the rats were sacrificed at day 1, 3, 5 and 7 post-infection, examined and removed vegetations from the valve as well as kidney and spleen for quantitative culture. The non surgical infection groups and non infection groups were also set in this experiment. Results Day one after infection, there were different size of valvular vegetations around cardiac catheterization, and even the wall vegetations were found in the serious MRSA infectious rats. IE in the rats were even worse along with increase of the concentration. Log CFU/g of vegetation in infection groups of 10^3 CFU/animal, 10^4 CFU/animal and 10^5 CFU/animal was 7.04 ± 1.38, 9.32 ± 1.22 and 9.78 ± 1.03, respectively. IE was most serious at day 3 post-infection, and the highest log CFU/g could reach 10.03 ± 0.71. Within 5- 7 days, the degrees of IE varied in different groups, and log CFU/g of vegetation in infection groups of 10^3 CFU/animal and 10^4 CFU/animal declined to 6.87 ± 0.63 and 8.23 ± 1.05, respectively. The trends of infection in the spleen and kidney were roughly the same as in the heart, but the colony counts were all lower than those of the heart. There were no vegetations in non surgical infection groups and non infection groups. Conclusion The model established by this method has high maneuverability, simplicity, stability, repeatability and success rate. It shows promise in evaluating the predictive efficacy of antibiotics for MRSA infected endocarditis.

关 键 词：耐甲氧西林金黄色葡萄球菌 心内膜炎 细菌性 疾病模型 动物 大鼠 

分 类 号：R-332[医药卫生] R542.41