鸭CD36基因表达对前列腺癌PC3细胞增殖的影响  

Effect of Duck CD36 Gene Expression on the Proliferation of PC3 Prostate Cancer Cells

作　　者：杨英仓[1,2] 李万贵[3] 王单单[3] 张依裕[3] 

机构地区：[1]贵州警官职业学院,贵阳550005 [2]贵州省道路交通事故鉴定工程技术研究中心,贵阳550005 [3]贵州大学动物科学学院高原山地动物遗传育种与繁殖教育部重点实验室,贵阳550025

出　　处：《基因组学与应用生物学》2016年第8期1950-1954,共5页Genomics and Applied Biology

基　　金：贵州省道路交通事故鉴定工程技术研究中心开放基金(黔道交鉴合G字[2015]10011号);贵州省科技厅社会攻关项目(黔科合G字[2014]4001号);国家自然科学基金(31160453)共同资助

摘　　要：为了探讨鸭CD36基因表达对前列腺癌PC3细胞增殖的效应，通过克隆鸭CD36基因CDS区，构建真核表达载体pEGFP-N3＋CD36＋His，空载体pEGFP—N3为对照，转染培养的前列腺癌PC3细胞。结果表明：前列腺癌PC3细胞均被pEGFP-N3＋CD36＋His和pEGFP—N3成功转染，转染效率均较高；通过检测转染48h的CD36＋His-tag融合蛋白表达量和细胞数量，CD36＋His—tag融合蛋白表达量为98．81pg／mL，pEGFP-N3＋CD36＋His组细胞数为0．72×106^个，pEGFP-N3组细胞数为0．61×106^个，pEGFP-N3＋CD36＋His组细胞数显著高于pEGFP-N3组。研究结果揭示，鸭CD36基因的表达对前列腺癌PC3细胞增殖和生长可能有促进作用。The purpose of this study was to investigate the effect of CD36 gene expression on the proliferation of prostate cancer PC3 cells. By cloning the CDS region of duck CD36 gene, the pEGFP-N3＋CD36＋His of eukaryotic expression vector was constructed, and the empty vector pEGFP-N3 for control group, transfected into the cultured PC3 prostate cancer cells. The results indicated that the PC3 prostate cancer cells were successfully transfection by two vectors, and had higher transfection efficiency. At 48 h, the CD36＋His-tag fusion protein expression and cell number were 98.81 pg/mL and 0.72× 106^ for pEGFP-N3 ＋CD36 ＋His group, respectively. However, the cell number of pEGFP-N3 group was 0.61× 106^. The cell number of pEGFP-N3 ＋CD36＋His group was significantly higher than pEGFP-N3 group. These findings suggested that the expression of duck CD36 gene might promote the proliferation and growth of PC3 prostate cancer cells.

关 键 词：CD36基因 前列腺癌PC3细胞 转染 增殖 

分 类 号：R737.25[医药卫生—肿瘤]