基于Miseq测序与碳平衡的生物滴滤床降解甲苯研究  

BIODEGRADATION MECHANISM OF A BIOTRICKLING FILTER TREATING TOLUENE BY MISEQ SEQUENCING AND CARBON EQUILIBRIUM ANALYSIS

作　　者：蒋海涛[1] 龙超[1] 翟建[1] 

机构地区：[1]南京大学环境学院污染控制与资源化研究国家重点实验室,南京210023

出　　处：《环境工程》2016年第8期114-119,共6页Environmental Engineering

摘　　要：在甲苯进气浓度为1 640 mg/m3,进气负荷为198.11 g/(m3·h),系统停留时间(EBRT)为30 s的条件下,生物滴滤床对甲苯的去除率达到84%,去除负荷为166.41 g/(m3·h)。碳平衡计算表明:22.27%的输入碳源为微生物所同化,59.52%的碳以CO2形式排出,仅有2.46%的碳溶于营养液中。在出口气体中,以CO2形式存在的碳占79.08%,其余则以甲苯形式存在。经Miseq-16S rRNA测序平台分析可知,该生物反应器中的优势菌属为假单胞菌、丛毛单胞菌和红球菌属,优势菌门为变形菌门、拟杆菌门和酸杆菌门。其中,变形菌门和拟杆菌门在反应器中所占比例在经过驯化之后显示出相反的变化趋势。A bench scale biotrickling filter reactor was steadily operated to treat 1 640 mg / m^3 of toluene. Nearly 84% toluene removal and the removal capacity of 166. 41 g /（ m^3·h） was achieved when the EBRT was at 30 s. Under the stable operating conditions,22. 27% of carbon resource was assimilated into biomass,and 59. 52% of carbon was degraded into CO2. Only2. 46% of carbon was dissolved in the nutrient solution. In the exhaust gas,carbon mass in CO2 accounted for 79. 08% and the rest carbon almost existed in the form of toluene. Miseq-16 S rRNA sequencing manifested that Pseudomonas,Comamonadaceae,and Rhodococcus were the three predominant genera in the bioreactor fed with toluene. From the perspective of phyla,Proteobacteria,Bacteroidetes and Acidobacteria were the three dominant phyla in the bioreactor,and among them,Proteobacteria and Bacteroidetes demonstrated opposite response to the introduction of toluene.

关 键 词：生物滴滤塔 甲苯 碳平衡 Miseq-16S RRNA 

分 类 号：X701[环境科学与工程—环境工程]