Peroxiredoxin 6在脑缺血再灌注损伤中的抗氧化和抗凋亡作用  

Antioxidant and antiapoptotic effects of Peroxiredoxin 6 in cerebral ischemia/reperfusion injury

作　　者：姜蓓蓓[1] 谭丽[1] 姚辉[1] 喻姗姗[1] 赵涌[1] 王顺和[1] 

机构地区：[1]重庆医科大学基础医学院病理教研室,重庆400016

出　　处：《重庆医科大学学报》2016年第8期781-786,共6页Journal of Chongqing Medical University

基　　金：国家自然科学基金面上资助项目(编号:81271460);国家自然科学基金青年资助项目(编号:81301125)

摘　　要：目的:探讨Peroxiredoxin 6(Prdx6)在脑缺血再灌注损伤中的影响及相关机制。方法:采用中脑动脉栓塞(middle cerebral artery occlusion,MCAO)模型诱导SD大鼠左侧脑皮质缺血再灌注,将大鼠随机(n=16)分为假手术组、MCAO组、Scramble组和Prdx6-si RNA组:前两组均不进行侧脑室注射,其中Sham组仅做手术切口,不插入线栓,MCAO组进行手术造模;后2组分别侧脑室注射8μl的乱序Prdx6-si RNA和Prdx6-si RNA再手术建模。测定各组神经功能学评分、脑含水量和脑梗死体积;HE和亚甲蓝染色法检测大脑皮质缺血再灌注区神经元的损伤情况;免疫印迹法检测脑组织中Prdx6及Caspase3蛋白的表达水平;最后,检测SOD、MDA活性并用TUNLE染色法探讨相关氧化、凋亡机制。结果:Prdx6-si RNA组与MCAO组相比,神经功能学评分、缺血侧脑含水量和脑梗死体积均明显增加(P=0.000);HE和亚甲蓝染色显示脑缺血区神经元损伤进一步加重(P=0.000);SOD活性进一步降低(P=0.000),MDA含量进一步增加(P=0.000);TUNLE染色阳性细胞率(P=0.000)和Caspase3蛋白表达水平(P<0.01)均进一步增高。而Scramble组与MCAO组比以上各指标均无明显统计学差异。结论:Prdx6在脑缺血再灌注损伤中起保护作用,此作用与其抗氧化和抗凋亡机制有关。Objective：To investigate the influence of Peroxiredoxin 6（Prdx6）on cerebral ischemia/-reperfusion（I/R）injury and its mechanism. Methods ：The middle cerebral artery occlusion（MCAO）model was used to induce SD rats＇ cerebral I/R on the left side. Rats（n =16） were randomly divided into Sham group,MCAO group,Scramble group and Prdx6-si RNA group;the former two groups did not receive lateral ventricle injection;Sham group only had surgery incision and line plug was not inserted;MCAO group received surgery;the latter two groups were respectively injected with 8 μl out-of-order Prdx6-si RNA and Prdx6-si RNA in lateral ventricle before surgery. In every group,nerve function score,brain water content and cerebral infarct volume were measured;HE and methylene blue staining method were used to detect the rats＇ brain cortex neurons after I/R injury. The expressions of Prdx6 and Caspase3 protein were detected by Western blot. SOD and MDA activities were assayed and TUNLE staining was used to detect the related mechanism of apoptosis and oxidation. Results：The neurological function score,lateral ischemic cerebral water content and cerebral infarction volume were significantly increased（P=0.000）in Prdx6- si RNA group than in MCAO group;HE and methylene blue staining showed further aggravate cerebral ischemic neuronal damage in Prdx6-si RNA group than in MCAO group（P=0.000）;SOD activity was further reduced（P=0.000）and MDA content was increased（P=0.000）in Prdx6-si RNA group than in MCAO group.TUNLE staining positive cells rate（P=0.000）and Caspase3 protein expression levels（P〈0.01）were further increased in Prdx6-si R NA group than in MCAO group. There was no significant statistical difference in the above indicators between Scramble and MCAO groups. Conclusion：Prdx6 has the protective effect on cerebral I/R injury,which relates with antioxidant and antiapoptotic mechanisms.

关 键 词：Prdx6-si RNA 脑缺血再灌注 氧化应激 抗凋亡 

分 类 号：R364.17[医药卫生—病理学] R363.22[医药卫生—基础医学]