基于血浆代谢组学分析金欣口服液治疗小鼠RSV肺炎的作用机制  

Mechanism Analysis of Jinxin Oral Liquid Against Respiratory Syncytial Virus Pneumonia Based on Plasma Metabonomics

作　　者：孟欣[1] 单进军[1] 谢彤[1] 徐建亚[1] 沈存思[1] 汪受传[1] 

机构地区：[1]南京中医药大学,江苏省儿童呼吸疾病(中医药)重点实验室,南京210023

出　　处：《中国实验方剂学杂志》2016年第17期71-76,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81373688,81303295);江苏省研究生培养创新工程项目(SJZZ15_0120)

摘　　要：目的：通过GC-MS技术分析BALB/c小鼠血浆内源性代谢产物的相对含量变化,阐明金欣口服液治疗呼吸道合胞病毒（RSV）肺炎的作用机制。方法：RSV滴鼻感染BALB/c小鼠建立RSV肺炎模型,首次滴鼻2 h后予金欣口服液（剂量27.6 g·kg-1·d-1）灌胃治疗,连续滴鼻3 d后各组处死5只小鼠取肺组织行组织病理学检查,连续给药7 d后采集各组小鼠的血浆,运用GC-MS检测（m/z 50-500）血浆内源性代谢物及其相对含量的变化,分析其可能参与的代谢通路。结果：RSV肺炎小鼠的血浆中共测得32种内源性代谢产物,其中乳酸、尿素、谷氨酰胺、葡萄糖、花生四烯酸和1,5-脱水山梨醇为差异代谢产物,主要与花生四烯酸、谷氨酰胺、丙酮酸的代谢通路有关。结论：金欣口服液治疗RSV肺炎可能与调节RSV感染导致的代谢紊乱相关。Objective： To detect the relative content changes of endogenous metabolites in plasma of BALB / c mice by GC-MS technology, and to explore the mechanism of Jinxin oral liquid against respiratory syncytial virus（ RSV） pneumonia in vivo. Method： BALB / c mice were challenged intranasally with RSV to establish the RSV pneumonia model, then intragastric administration of Jinxin oral liquid with dose of 27. 6g·kg-1·d-1. After 3 days of inoculation,five mice in each group were hanged and inferior lobe of right lung were collected to take histopathological examination. After continuously given Jinxin oral liquid for 7 days,plasma were collected to detect the endogenous metabolites by GC-MS（ m / z 50-500） and possible metabolic pathways concerned were analyzed. Result： Thirty-two endogenous metabolites in plasma were detected,arachidonic acid,glutamine,lactic acid,D-glucose,urea and 1,5-anhydro-D-sorbitol were differential metabolites among them. Metabolic pathways of archidonic acid,glutamine and pyruvate were the most concerned. Conclusion： The antiviral effect of Jinxin oral liquid against RSV pneumonia may be related to regulation of metabolic disorder caused by RSV.

关 键 词：气相色谱-质谱联用 金欣口服液 血浆 呼吸道合胞病毒性肺炎 

分 类 号：R969.1[医药卫生—药理学] R284.1[医药卫生—药学]