长链非编码RNAPANDAR在肾癌中的表达分析及临床意义  

Expression and clinical significance of long non-coding RNA PANDAR in renal cell carcinoma

作　　者：金露[1,2,3] 李一帆[1,2,3] 何韬[2,3] 胡佳[2,3] 刘家驹[2,3] 桂耀庭[2,3] 杨尚琪[2,3] 毛向明[2,3] 来永庆[2,3] 

机构地区：[1]安徽医科大学第二临床医学院,合肥230032 [2]北京大学深圳医院泌尿外科,深圳518036 [3]广东省男性生殖与遗传重点实验室,深圳518036

出　　处：《安徽医科大学学报》2016年第9期1342-1345,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81101922);深圳市科技研发资金知识创新计划基础研究项目(编号:JCYJ20150403091443304);广东省重点医学专科经费

摘　　要：目的检测长链非编码RNA PANDAR在肾癌及癌旁组织中的表达水平,探讨其在肾癌中的临床意义。方法提取48对组织(肾癌及对应癌旁组织)中的总RNA,经逆转录获得c DNA后通过实时荧光定量聚合酶链式反应(qRTPCR)方法检测PANDAR表达量,分析正常组织与肾癌组织中的表达差异,探讨其表达水平与患者临床特征之间的联系。结果肾癌组织中PANDAR的表达水平明显低于配对癌旁正常组织(P<0.001),标本中共有38例(79.17%)肾癌标本PANDAR表达下调,癌组织中PANDAR表达与患者TNM分期和AJCC分级相关,与患者年龄、性别及肾癌病理类型无明显相关性(P>0.05),PANDAR低表达组患者生存率低于高表达组患者(P<0.05)。结论 PANDAR在肾癌组织中表达明显下调,且和肾癌TNM、AJCC分级和预后相关,提示其有作为肾癌标志物应用于临床的可能。Objective To detect the expression level of long non-coding RNA（ lncRNA） PANDAR in paired renal cell carcinoma（ RCC） and normal tissues and explore PANDAR＇ s clinical significance in renal cell carcinoma.Methods Total RNA was isolated from 48 paired tissues,and the total RNA was used to get c DNA by performing reverse transcription. Then real time quantitative PCR（ RT-q PCR） was performed to detect the expression of PANDAR. Statistical analysis was performed to explore the differences of PANDAR＇ s expression level and the relationships between the expression of PANDAR and clinical characteristics. Results The expression level of lncRNA PANDAR was significantly lower in RCC tissues（ P〈 0. 001） than paired normal tissues and PANDAR was downregulated in 38（ 79. 17%） RCC tissues. The results showed that the expression of PANDAR was associated with the TNM and AJCC stage. However,no relationships were found between the expression of PANDAR and patients＇ age,sex and pathological types of RCC. Overall survival rate of patients with lower expression of PANDAR was significantly lower than patients with higher expression of PANDAR（ P〈 0. 05）. Conclusion In RCC tissues PANDAR is down-regulated,and the expression of PANDAR is associated with prognosis,TNM and AJCC stage of RCC,which indicates that it may be involved in the tumorigenesis and development of RCC.

关 键 词：肾癌 肿瘤分期 长链非编码RNA PANDAR 

分 类 号：R737.11[医药卫生—肿瘤]