MicroRNA-27a对U251胶质瘤细胞的影响  

The effect of microRNA-27a on U251 glioma cells

作　　者：刘国军[1] 张俊[1] 范永春[1] 王庆锁[1] 李伟[1] 

机构地区：[1]黑龙江省农垦总医院康复科,哈尔滨150088

出　　处：《实用肿瘤学杂志》2016年第4期346-350,共5页Practical Oncology Journal

摘　　要：目的探讨microRNA-27a(miR-27a)对U251胶质瘤细胞的影响。方法采用体外瞬时转染的方法过表达或抑制U251细胞中的miR-27a,MTT方法检测细胞的增殖情况,Transwell侵袭试验测定U251胶质瘤细胞的侵袭能力,Real-time PCR方法测定U251细胞中miR-27a和PPARγ的含量。结果抑制miR-27a可降低U251胶质瘤细胞增殖能力,减弱U251胶质瘤细胞侵袭能力,增加PPARγ的含量;过表达miR-27a促进U251胶质瘤细胞增殖能力,提高U251胶质瘤细胞侵袭能力,减少PPARγ的含量。结论抑制miR-27a有利于降低U251胶质瘤细胞的增殖能力和侵袭能力。Objective To discuss the effect of microRNA -27a on U251 glioma cells.Methods Over-expression or inhibition of miR -27a in U251 glioma cells were done by transient transfection of miR -27a mim-ics or AMO-27a in vitro.Cell viability was detected by MTT assay .Invasion ability of U251 was detected by tr-answell invasion assay.The level of miR-27a and PPARγwere detected by real -time PCR.Results Under in-hibition of miR-27a condition,the proliferation and invasiveness of U 251 glioma cells were decreased .The level of PPARγwas significantly increased ,whereas the level of miR-27 a was decreased .Overexpression of miR-27 a increased the proliferation and invasiveness of U 251 glioma cells and decreased the level of PPARγ.Conclusion Inhibition of miR-27a is benefit for inhibiting the proliferation and invasiveness of U 251 glioma cells.

关 键 词：微小RNA-27 a 胶质瘤 PPARΓ 增殖 侵袭 

分 类 号：R739.41[医药卫生—肿瘤]