大麦SSR标记遗传多样性及连锁不平衡分析  

Genetic Diversity and Linkage Disequilibrium Analysis of Barley Using SSR Markers

作　　者：赖勇[1] 王晋民[1] 任龙[2] 朱惠琴[1] 马辉[1] 王金贵[1] 巨霞[1] 李宗仁[1] 

机构地区：[1]青海大学农牧学院农林系,青海西宁810016 [2]青海大学现代教育技术中心,青海西宁810016

出　　处：《核农学报》2016年第10期1889-1897,共9页Journal of Nuclear Agricultural Sciences

基　　金：青海大学博士启动基金项目

摘　　要：为确定不同青稞亲本材料间的遗传差异及连锁不平衡水平,以56个SSR分子标记对88份大麦进行多态性扫描。结果表明,88份材料中共检测出160个等位变异,平均每个标记2.857个,变幅为2~7。供试材料间的遗传相似系数为0.497~0.970,平均为0.761。基于多态性较好、基因多样性值较高的53个SSR标记分析结果,88份材料被分成2个类别。主坐标分析将青稞材料分成2类,分别包含39和47份材料,2份西藏品种ZDM5200和ZDM5457所属类别不明确。群体遗传结构分析将88份材料也分成2个亚群,分别包含40份和48份材料,与聚类分析和主坐标分析的结果较一致。1 378个SSR位点成对组合中,不论共线性组合还是非共线性组合,都存在一定程度的连锁不平衡（LD）。D＇统计概率（P〈0.01）支持的LD成对位点179个,占全部位点组合的12.99%,D＇平均值为0.56,较高水平的LD成对位点（D〉0.5）主要集中于除1H外的其余6个连锁群上。本研究结果为今后青稞杂交组合配置、有利基因发掘和标记辅助育种提供了理论依据。In order to get an insight of the genetic differences and the levels of linkage disequilibrium among different hulless barley material,88 barley materials were scaned with 56 SSR marker. A total of 160 alleles were detected from these accessions,with an average of 2. 857 alleles per marker,ranged from 2 to 7. The genetic similarity coefficient among them was from 0. 497 to 0. 970,with an average of 0. 761. Based on the results of 53 SSR makers with high polymorphism information content and gene diversity,88 accessions were divided into two subgroups,with principal coordinate analysis,each group included 39 and 47 materials,respectively. But two Tibet materials ZDM5200 and ZDM5457 could not be clustered clearly. These 88 barley materials could also be divided into 2 subgroups by population structure analysis,including 40 and 48 accessions,respectively,which was basically identical with the results of clustering and principal coordinate analysis. Some significant linkage disequilibrium（ LD） were detected among linked loci and unlinked loci pairs based on the results of 53 SSR analysis. One hundred and seventy-nine out of 1 378 loci pairs（ 12. 99%） had significant LD（ P〈0. 01） with an average D＇ value of 0. 56. The loci pairs of LD with high level（ D＇〉0. 5） were mainly distributed across six chromosomes,except 1H. These results would provide useful information for the hybridization combinations,exploitation of favorable genes and marker-assistant selection of hulless barley.

关 键 词：青稞 SSR标记 遗传多样性 群体结构 连锁不平衡 

分 类 号：S512.3[农业科学—作物学]