茶树抗寒基因CsCBF1与CsICE1低温下的表达分析  

Expression Analysis of Cold-resistant CsCBF1 and CsICE1 Genes From Tea Plant Under Low Temperature

作　　者：林郑和[1] 钟秋生[1] 单睿阳[1] 陈常颂[1] 陈志辉[1] 游小妹[1] 

机构地区：[1]福建省农业科学院茶叶研究所,福建福安355015

出　　处：《核农学报》2016年第9期1693-1698,共6页Journal of Nuclear Agricultural Sciences

基　　金：福建省公益类专项(2015R1012-4);国家茶叶产业技术体系(nycytx-23);中国乌龙茶产业协同创新中心(培育)专项(2013-51)

摘　　要：为探明茶树抗寒转录因子基因CsCBF1与CsICE1的表达特性,以茶树幼苗为试验材料,根据其他已知植物的抗寒转录因子基因CBF1与ICE1序列,通过同源克隆法获得茶树中相对应的同源基因CsCBF1与CsICE1的保守区域,长度分别为456 bp与643 bp,经比对确定为CBF与ICE转录因子家族基因中CsCBF1与CsICE1型基因,因此命名为CsCBF1与CsICE1。荧光定量PCR分析CsCBF1与CsICE1基因在冷胁迫条件下的表达情况,发现其在4℃处理8 h时表达量最高;在茶树叶片中表达量显著高于根、茎、花。低温下CsCBF1与CsICE1基因表达显著增加,并且在抗寒性较强的种质(0306I和黄旦)中的表达显著高于抗寒性相对较弱的种质(0306F和0306D)。本研究结果为进一步探究CsCBF1与CsICE1基因在茶树耐低温胁迫过程中基因表达的调控机制奠定了基础。In order to understand the expression character of cold-resistant CsCBF1 and CslCE1 genes from tea plant, the corresponding homologous of the CsCBF1 and CslCElgene were cloned by homology cloning method in using a material according to the sequences of cold tolerance gene CBF1 and ICE1 had been reported in other plant species. The lengths of CsCBF1 and CslCElgene were 456 bp and 643 bp, respectively. Based on the alignment results compared with other reported CBF and ICE genes, the CsCBF and CslCE gene were found to belongs to the CBF1 and ICE1 familiy and named as CsCBF1 and CslCE1, respectively. By real-time quantitative PCR, the expression level of the CsCBF1 and CslCE1 gene were analyzed in the cold condition were analyzed and found that the expression of both genes reached peak at eight hours after 4℃ treatment. The result showed that CsCBF1 and CslCE1 was expressed the highest in leaves among all of the tissues, including roots, stems, leaves and flowers. In addition, CsCBF1 and CslCE1 gene was induced by cold stress, and the both gene expression level of both gene in tea varieties 0306I and Huangdan were higher than in tea varieties 0306F and 0306D. These results may provide basis for further study of regulation function of gene expression CsCBF1 and CslCE1 in during low temperature stress.

关 键 词：茶树 抗寒 CsCBF1 CsICE1 表达 

分 类 号：S571.1[农业科学—茶叶生产加工]